{
    "organism": "Lacticaseibacillus chiayiensis",
    "built_at": "2026-10-07T11:29:50+05:30",
    "review_status": "unreviewed",
    "generator": "CultureScope 1.0.0",
    "disclaimer": "Aggregated from published literature. Unreviewed. Not a substitute for a validated SOP or an accredited reference laboratory.",
    "taxonomy": {
        "taxon": "Lacticaseibacillus chiayiensis",
        "lineage": "cellular organisms; Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae; Lacticaseibacillus",
        "rank": "species",
        "taxid": "2100821",
        "taxonomy_url": "https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=2100821",
        "gram_prior": {
            "value": "Gram-positive",
            "basis": "Inferred from NCBI Taxonomy lineage"
        },
        "accepted": "Lacticaseibacillus chiayiensis",
        "gbif_status": "ACCEPTED",
        "gbif_name": "Lacticaseibacillus chiayiensis Chiayiensis, 2020"
    },
    "fields": {
        "media": {
            "label": "Media used",
            "multi": true,
            "items": [
                {
                    "key": "mrs_agar_broth",
                    "value": "MRS agar/broth",
                    "score": 7.35,
                    "sources": [
                        "pmc"
                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "LAB were routinely grown in the study in MRS broth at 37 degC for 24 h and reinoculated in fresh MRS broth at 37 degC.",
                            "source": "pmc",
                            "title": "Genomic and in-vitro characteristics of a novel strain Lacticaseibacillus chiayiensis AACE3 isolated from fermented blueberry",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235500/",
                            "year": "2023",
                            "id": "PMC10235500"
                        },
                        {
                            "sentence": "Briefly, L. chiayiensis AACE3 was inoculated in MRS broth and cultured at 37 degC for 24 h.",
                            "source": "pmc",
                            "title": "Genomic and in-vitro characteristics of a novel strain Lacticaseibacillus chiayiensis AACE3 isolated from fermented blueberry",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235500/",
                            "year": "2023",
                            "id": "PMC10235500"
                        },
                        {
                            "sentence": "For the individual colonies used for routine use, the strain was grown and passaged in lactic acid bacteria selective medium (MRS) broth at 37 degC for 24 h.",
                            "source": "pmc",
                            "title": "Effects of Dietary Supplementation of Lacticaseibacillus Chiayiensis AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao Chicks",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525887/",
                            "year": "2023",
                            "id": "PMC10525887"
                        },
                        {
                            "sentence": "For the isolation of lactic acid bacterial strains, the serially diluted milk samples were spread on an MRS agar plate (Difco, Becton & Dickinson, Sparks, MD, United States) and incubated at 37 degC for 48 h under anaerobic conditions.",
                            "source": "pmc",
                            "title": "Complete Genome Sequencing and Comparative Genomics of Three Potential Probiotic Strains, Lacticaseibacillus casei FBL6, Lacticaseibacillus chiayiensis FBL7, and Lacticaseibacillus zeae FBL8",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777060/",
                            "year": "2022",
                            "id": "PMC8777060"
                        },
                        {
                            "sentence": "The different individual colonies were randomly selected according to morphological differences and purified on MRS broth (Difco).",
                            "source": "pmc",
                            "title": "Complete Genome Sequencing and Comparative Genomics of Three Potential Probiotic Strains, Lacticaseibacillus casei FBL6, Lacticaseibacillus chiayiensis FBL7, and Lacticaseibacillus zeae FBL8",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777060/",
                            "year": "2022",
                            "id": "PMC8777060"
                        },
                        {
                            "sentence": "In brief, preserved vaginal swab samples were freeze-thawed, diluted, and spread onto agar media, including modified MRS agar containing 0.12 g/L of bromocresol purple (Sigma Aldrich, Steinheim, Germany) and 0.1% L-cystine HCl (Sigma Aldrich, Germany), as well as Rogosa SL agar.",
                            "source": "pmc",
                            "title": "Clinical Potential of Novel Microbial Therapeutic LP51 Based on Xerosis-Microbiome Index",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11639849/",
                            "year": "2024",
                            "id": "PMC11639849"
                        }
                    ],
                    "inferred": false
                },
                {
                    "key": "brain_heart_infusion_bhi_",
                    "value": "Brain heart infusion (BHI)",
                    "score": 4.04,
                    "sources": [
                        "pmc"
                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "The bacterial strain was routinely grown in BHI medium (Oxoid, Basingstone, UK) supplemented with 3% ( w/v ) NaCl (Solaibao, Beijing, China) at an optimal temperature of 28 degC.",
                            "source": "pmc",
                            "title": "Whole-Genome Sequence Analysis, Probiotic Potential, and Safety Assessment of the Marine Bacterium Paraliobacillus zengyii CGMCC1.16464",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12112907/",
                            "year": "2025",
                            "id": "PMC12112907"
                        },
                        {
                            "sentence": "The activated culture was then transferred at a 1:100 dilution into fresh BHI media containing 3% ( w / v ) NaCl and further cultured with shaking for 6 to 8 h until the logarithmic growth phase was reached (OD 600 = 0.6).",
                            "source": "pmc",
                            "title": "Whole-Genome Sequence Analysis, Probiotic Potential, and Safety Assessment of the Marine Bacterium Paraliobacillus zengyii CGMCC1.16464",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12112907/",
                            "year": "2025",
                            "id": "PMC12112907"
                        },
                        {
                            "sentence": "E. coli PET-24a and S. aureus 25923 were cultured in BHI medium at 37 degC.",
                            "source": "pmc",
                            "title": "Whole-Genome Sequence Analysis, Probiotic Potential, and Safety Assessment of the Marine Bacterium Paraliobacillus zengyii CGMCC1.16464",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12112907/",
                            "year": "2025",
                            "id": "PMC12112907"
                        }
                    ],
                    "inferred": false
                },
                {
                    "key": "luria_bertani_lb_",
                    "value": "Luria-Bertani (LB)",
                    "score": 1.86,
                    "sources": [
                        "pmc",
                        "crossref"
                    ],
                    "n_sources": 2,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "Then, the indicated pathogens (100 μL, 1 × 10 7 CFU/mL) were mixed with 5 mL of semisolid LB agar medium and quickly poured onto the surface of solid LB agar medium.",
                            "source": "pmc",
                            "title": "Genomic and in-vitro characteristics of a novel strain Lacticaseibacillus chiayiensis AACE3 isolated from fermented blueberry",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235500/",
                            "year": "2023",
                            "id": "PMC10235500"
                        },
                        {
                            "sentence": "Planktonic persisters of two clinical strains of P. aeruginosa (PaCF1 and PaCF4) were obtained following two different procedures: (i) exposing stationary-phase cultures to cyanide m-chlorophenylhydrazone (CCCP) in LB medium; (ii) incubating stationary-phase cultures with high doses of tobramycin (128-fold MIC) in ASM.",
                            "source": "crossref",
                            "title": "Anti-Persisters Activity of Lacticaseibacillus rhamnosus Culture Filtrates against Pseudomonas aeruginosa in Artificial Sputum Medium",
                            "url": "https://doi.org/10.3390/ijms25137113",
                            "year": "2024",
                            "id": "doi:10.3390/ijms25137113"
                        }
                    ],
                    "inferred": false
                }
            ],
            "confidence": "low",
            "conflict": null,
            "review": "unreviewed"
        },
        "temperature": {
            "label": "Optimum temperature for growth",
            "multi": false,
            "items": [
                {
                    "key": "p28",
                    "value": "28 °C",
                    "score": 1.4,
                    "sources": [
                        "pmc"
                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "The bacterial strain was routinely grown in BHI medium (Oxoid, Basingstone, UK) supplemented with 3% ( w/v ) NaCl (Solaibao, Beijing, China) at an optimal temperature of 28 degC.",
                            "source": "pmc",
                            "title": "Whole-Genome Sequence Analysis, Probiotic Potential, and Safety Assessment of the Marine Bacterium Paraliobacillus zengyii CGMCC1.16464",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12112907/",
                            "year": "2025",
                            "id": "PMC12112907"
                        }
                    ],
                    "inferred": false
                },
                {
                    "key": "p37",
                    "value": "37 °C",
                    "score": 1.38,
                    "sources": [
                        "pmc"
                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "Briefly, L. chiayiensis AACE3 was inoculated in MRS broth and cultured at 37 degC for 24 h.",
                            "source": "pmc",
                            "title": "Genomic and in-vitro characteristics of a novel strain Lacticaseibacillus chiayiensis AACE3 isolated from fermented blueberry",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235500/",
                            "year": "2023",
                            "id": "PMC10235500"
                        }
                    ],
                    "inferred": false
                }
            ],
            "confidence": "low",
            "conflict": "Sources disagree: \"28 °C\" (score 1.4) versus \"37 °C\" (score 1.38). Both are shown; a reviewer should decide.",
            "review": "unreviewed"
        },
        "time": {
            "label": "Time required to grow",
            "multi": false,
            "items": [
                {
                    "key": "hrs_0_48",
                    "value": "0-48 h",
                    "score": 6.88,
                    "sources": [
                        "pmc",
                        "openalex"
                    ],
                    "n_sources": 2,
                    "confidence": "moderate",
                    "evidence": [
                        {
                            "sentence": "Cultures were incubated at 37 degC for 0-48 h under varying conditions-namely aerobic, anaerobic, and microaerophilic chambers (Baker Ruskin, Rotherham, UK).",
                            "source": "pmc",
                            "title": "Clinical Potential of Novel Microbial Therapeutic LP51 Based on Xerosis-Microbiome Index",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11639849/",
                            "year": "2024",
                            "id": "PMC11639849"
                        },
                        {
                            "sentence": "LAB were routinely grown in the study in MRS broth at 37 degC for 24 h and reinoculated in fresh MRS broth at 37 degC.",
                            "source": "pmc",
                            "title": "Genomic and in-vitro characteristics of a novel strain Lacticaseibacillus chiayiensis AACE3 isolated from fermented blueberry",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235500/",
                            "year": "2023",
                            "id": "PMC10235500"
                        },
                        {
                            "sentence": "Briefly, L. chiayiensis AACE3 was inoculated in MRS broth and cultured at 37 degC for 24 h.",
                            "source": "pmc",
                            "title": "Genomic and in-vitro characteristics of a novel strain Lacticaseibacillus chiayiensis AACE3 isolated from fermented blueberry",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235500/",
                            "year": "2023",
                            "id": "PMC10235500"
                        },
                        {
                            "sentence": "Then, the plates were transferred to a constant-temperature incubator at 37 degC for 24 h to observe the bacterial inhibitory activity.",
                            "source": "pmc",
                            "title": "Genomic and in-vitro characteristics of a novel strain Lacticaseibacillus chiayiensis AACE3 isolated from fermented blueberry",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235500/",
                            "year": "2023",
                            "id": "PMC10235500"
                        },
                        {
                            "sentence": "For the individual colonies used for routine use, the strain was grown and passaged in lactic acid bacteria selective medium (MRS) broth at 37 degC for 24 h.",
                            "source": "pmc",
                            "title": "Effects of Dietary Supplementation of Lacticaseibacillus Chiayiensis AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao Chicks",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525887/",
                            "year": "2023",
                            "id": "PMC10525887"
                        },
                        {
                            "sentence": "The strain exhibited exponential growth, reaching 8.8 log CFU/mL within 24 h, and demonstrated strong acid tolerance with survival rates of 54% at pH 3.0, 99% at pH 3.5, and 100% at pH 7.",
                            "source": "openalex",
                            "title": "In vitro evaluation of Zymomonas mobilis UFPEDA 363 as a probiotic: safety profile, antagonism, and simulated gastrointestinal resistance",
                            "url": "https://doi.org/10.1590/0001-3765202620250788",
                            "year": "2026",
                            "id": "https://openalex.org/W7169801540"
                        }
                    ],
                    "inferred": false
                },
                {
                    "key": "hrs_336_336",
                    "value": "14 d",
                    "score": 1.4,
                    "sources": [
                        "openalex"
                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "The starter culture was rejuvenated in coconut water medium and incubated at 30 degC for 14 days.",
                            "source": "openalex",
                            "title": "Microbial diversity of bacterial cellulose starter culture revealed by 16S rRNA gene amplicon sequencing",
                            "url": "https://doi.org/10.13057/biodiv/d270614",
                            "year": "2026",
                            "id": "https://openalex.org/W7201951902"
                        }
                    ],
                    "inferred": false
                },
                {
                    "key": "hrs_504_504",
                    "value": "21 d",
                    "score": 0.78,
                    "sources": [
                        "pmc"
                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "The chicks were weighed after 21 days, and the PC, LC6 and LC7 groups were found to be 11.28 g, 4.56 g and 14.69 g heavier than the NC group, respectively.",
                            "source": "pmc",
                            "title": "Effects of Dietary Supplementation of Lacticaseibacillus Chiayiensis AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao Chicks",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525887/",
                            "year": "2023",
                            "id": "PMC10525887"
                        }
                    ],
                    "inferred": false
                },
                {
                    "key": "hrs_72_72",
                    "value": "3 d",
                    "score": 0.78,
                    "sources": [
                        "pmc"
                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "Moreover, the transcription level of LOR was remarkably enhanced under treatment with 3% of LP51 culture filtrate ( Figure 3 D).",
                            "source": "pmc",
                            "title": "Clinical Potential of Novel Microbial Therapeutic LP51 Based on Xerosis-Microbiome Index",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11639849/",
                            "year": "2024",
                            "id": "PMC11639849"
                        }
                    ],
                    "inferred": false
                },
                {
                    "key": "hrs_6_8",
                    "value": "6-8 h",
                    "score": 0.78,
                    "sources": [
                        "pmc"
                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "The activated culture was then transferred at a 1:100 dilution into fresh BHI media containing 3% ( w / v ) NaCl and further cultured with shaking for 6 to 8 h until the logarithmic growth phase was reached (OD 600 = 0.6).",
                            "source": "pmc",
                            "title": "Whole-Genome Sequence Analysis, Probiotic Potential, and Safety Assessment of the Marine Bacterium Paraliobacillus zengyii CGMCC1.16464",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12112907/",
                            "year": "2025",
                            "id": "PMC12112907"
                        }
                    ],
                    "inferred": false
                }
            ],
            "confidence": "moderate",
            "conflict": null,
            "review": "unreviewed"
        },
        "colony": {
            "label": "Colony morphology",
            "multi": false,
            "items": [],
            "confidence": "none",
            "conflict": null,
            "review": "unreviewed"
        },
        "gram": {
            "label": "Gram character",
            "multi": false,
            "items": [
                {
                    "key": "gram_positive",
                    "value": "Gram-positive",
                    "score": 7.98,
                    "sources": [
                        "pmc",
                        "europepmc"
                    ],
                    "n_sources": 2,
                    "confidence": "moderate",
                    "evidence": [
                        {
                            "sentence": "Antibacterial activity The antibacterial activity and antimicrobial spectrum of L. chiayiensis AACE3 against gram-positive (2) and gram-negative (6) indicators were determined by the modified Oxford cup double-layer plate method ( Voulgari et al., 2010 ).",
                            "source": "pmc",
                            "title": "Genomic and in-vitro characteristics of a novel strain Lacticaseibacillus chiayiensis AACE3 isolated from fermented blueberry",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235500/",
                            "year": "2023",
                            "id": "PMC10235500"
                        },
                        {
                            "sentence": "The results indicate that AACE3 has broad-spectrum antibacterial activity against common gram-positive and gram-negative pathogens.",
                            "source": "pmc",
                            "title": "Genomic and in-vitro characteristics of a novel strain Lacticaseibacillus chiayiensis AACE3 isolated from fermented blueberry",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235500/",
                            "year": "2023",
                            "id": "PMC10235500"
                        },
                        {
                            "sentence": "Four rod-shaped, non-motile, non-spore-forming, facultative anaerobic, Gram-stain-positive lactic acid bacteria, designated as EB0058 T , SCR0080, LD0937 T and SCR0063 T , were isolated from different corn and grass silage samples.",
                            "source": "europepmc",
                            "title": "&lt;i&gt;Lacticaseibacillus parahuelsenbergensis&lt;/i&gt; sp. nov., &lt;i&gt;Lacticaseibacillus styriensis&lt;/i&gt; sp. nov. and &lt;i&gt;Lacticaseibacillus zeae&lt;/i&gt; subsp. &lt;i&gt;silagei&lt;/i&gt; subsp. nov., isolated from different grass and corn silage.",
                            "url": "https://doi.org/10.1099/ijsem.0.006441",
                            "year": "2024",
                            "id": "MED:38954457 doi:10.1099/ijsem.0.006441"
                        },
                        {
                            "sentence": "Inferred from NCBI Taxonomy lineage: cellular organisms; Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae; Lacticaseibacillus",
                            "source": "ncbi_taxonomy",
                            "title": "NCBI Taxonomy lineage",
                            "url": "https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=2100821",
                            "year": "2026",
                            "id": "lineage"
                        }
                    ],
                    "inferred": false
                },
                {
                    "key": "gram_negative",
                    "value": "Gram-negative",
                    "score": 4.59,
                    "sources": [
                        "pmc"
                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "Antibacterial activity The antibacterial activity and antimicrobial spectrum of L. chiayiensis AACE3 against gram-positive (2) and gram-negative (6) indicators were determined by the modified Oxford cup double-layer plate method ( Voulgari et al., 2010 ).",
                            "source": "pmc",
                            "title": "Genomic and in-vitro characteristics of a novel strain Lacticaseibacillus chiayiensis AACE3 isolated from fermented blueberry",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235500/",
                            "year": "2023",
                            "id": "PMC10235500"
                        },
                        {
                            "sentence": "The results indicate that AACE3 has broad-spectrum antibacterial activity against common gram-positive and gram-negative pathogens.",
                            "source": "pmc",
                            "title": "Genomic and in-vitro characteristics of a novel strain Lacticaseibacillus chiayiensis AACE3 isolated from fermented blueberry",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235500/",
                            "year": "2023",
                            "id": "PMC10235500"
                        },
                        {
                            "sentence": "Moreover, AACE3 showed robust antibacterial activity against gram-negative indicators such as Klebsiella pneumoniae ATCC 10031 (inhibitory zone diameter of 19.46 ± 0.28 mm), Salmonella enteritidis CMCC (B) 50335 (inhibitory zone diameter of 18.22 ± 0.14 mm) and Escherichia coli CMCC (B) 44102 (inhibitory zone diameter of 17.96 ± 0.22 mm).",
                            "source": "pmc",
                            "title": "Genomic and in-vitro characteristics of a novel strain Lacticaseibacillus chiayiensis AACE3 isolated from fermented blueberry",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235500/",
                            "year": "2023",
                            "id": "PMC10235500"
                        }
                    ],
                    "inferred": false
                }
            ],
            "confidence": "moderate",
            "conflict": null,
            "review": "unreviewed"
        },
        "biochem": {
            "label": "Biochemical tests for identification",
            "multi": true,
            "items": [
                {
                    "key": "16s_rrna_gene_sequencing",
                    "value": "16S rRNA gene sequencing",
                    "score": 15.48,
                    "sources": [
                        "pmc",
                        "europepmc",
                        "openalex"
                    ],
                    "n_sources": 3,
                    "confidence": "high",
                    "evidence": [
                        {
                            "sentence": "These strains were identified via 16S rRNA gene sequencing with 27F/1492R primer pairs.",
                            "source": "pmc",
                            "title": "Complete Genome Sequencing and Comparative Genomics of Three Potential Probiotic Strains, Lacticaseibacillus casei FBL6, Lacticaseibacillus chiayiensis FBL7, and Lacticaseibacillus zeae FBL8",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777060/",
                            "year": "2022",
                            "id": "PMC8777060"
                        },
                        {
                            "sentence": "16S rRNA Gene-Sequencing-Based Identification of Isolated Probiotics The probiotic strain was identified through 16S rRNA gene-sequencing technology.",
                            "source": "pmc",
                            "title": "Clinical Potential of Novel Microbial Therapeutic LP51 Based on Xerosis-Microbiome Index",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11639849/",
                            "year": "2024",
                            "id": "PMC11639849"
                        },
                        {
                            "sentence": "The isolated strains were identified using 16S rRNA gene sequencing.",
                            "source": "pmc",
                            "title": "Clinical Potential of Novel Microbial Therapeutic LP51 Based on Xerosis-Microbiome Index",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11639849/",
                            "year": "2024",
                            "id": "PMC11639849"
                        },
                        {
                            "sentence": "The isolated strains were characterized using a polyphasic approach and EB0058 T and SCR0080 were identified as Lacticaseibacillus zeae by 16S rRNA gene sequence analysis.",
                            "source": "europepmc",
                            "title": "&lt;i&gt;Lacticaseibacillus parahuelsenbergensis&lt;/i&gt; sp. nov., &lt;i&gt;Lacticaseibacillus styriensis&lt;/i&gt; sp. nov. and &lt;i&gt;Lacticaseibacillus zeae&lt;/i&gt; subsp. &lt;i&gt;silagei&lt;/i&gt; subsp. nov., isolated from different grass and corn silage.",
                            "url": "https://doi.org/10.1099/ijsem.0.006441",
                            "year": "2024",
                            "id": "MED:38954457 doi:10.1099/ijsem.0.006441"
                        },
                        {
                            "sentence": "Isolates were identified by the homology of the 16S rRNA gene sequence.",
                            "source": "europepmc",
                            "title": "Associations between intestinal lactic acid bacteria species and feeding habits of zoo animals.",
                            "url": "https://doi.org/10.20517/mrr.2024.08",
                            "year": "2024",
                            "id": "MED:39421257 doi:10.20517/mrr.2024.08"
                        },
                        {
                            "sentence": "The aim of this research is to characterize the bacterial diversity and community composition of a BC starter culture using 16S rRNA gene amplicon sequencing and to complement the molecular data with culture-based enumeration.",
                            "source": "openalex",
                            "title": "Microbial diversity of bacterial cellulose starter culture revealed by 16S rRNA gene amplicon sequencing",
                            "url": "https://doi.org/10.13057/biodiv/d270614",
                            "year": "2026",
                            "id": "https://openalex.org/W7201951902"
                        }
                    ],
                    "inferred": false
                },
                {
                    "key": "whole_genome_sequencing_ani",
                    "value": "Whole-genome sequencing / ANI",
                    "score": 11.03,
                    "sources": [
                        "pmc",
                        "europepmc",
                        "openalex"
                    ],
                    "n_sources": 3,
                    "confidence": "high",
                    "evidence": [
                        {
                            "sentence": "Analysis of Whole-Genome Sequencing Results of the Strain The primary genomic characteristics of the LP51 strain are illustrated in Figure 1 .",
                            "source": "pmc",
                            "title": "Clinical Potential of Novel Microbial Therapeutic LP51 Based on Xerosis-Microbiome Index",
                            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11639849/",
                            "year": "2024",
                            "id": "PMC11639849"
                        },
                        {
                            "sentence": "Based on whole-genome sequence-based characterization, EB0058 T and SCR0080 were separated into a distinct clade from Lacticaseibacillus zeae DSM 20178 T , together with CECT9104 and UD2202, whose genomic sequences are available from NCBI GenBank.",
                            "source": "europepmc",
                            "title": "&lt;i&gt;Lacticaseibacillus parahuelsenbergensis&lt;/i&gt; sp. nov., &lt;i&gt;Lacticaseibacillus styriensis&lt;/i&gt; sp. nov. and &lt;i&gt;Lacticaseibacillus zeae&lt;/i&gt; subsp. &lt;i&gt;silagei&lt;/i&gt; subsp. nov., isolated from different grass and corn silage.",
                            "url": "https://doi.org/10.1099/ijsem.0.006441",
                            "year": "2024",
                            "id": "MED:38954457 doi:10.1099/ijsem.0.006441"
                        },
                        {
                            "sentence": "Through whole-genome sequence analysis, the strain TISTR 2593 was identified as Lacticaseibacillus paracasei.",
                            "source": "openalex",
                            "title": "Evaluating the safety and efficacy of Lacticaseibacillus paracasei TISTR 2593 as a therapeutic probiotic for obesity prevention",
                            "url": "https://doi.org/10.3389/fmicb.2025.1501395",
                            "year": "2025",
                            "id": "https://openalex.org/W4409074428"
                        },
                        {
                            "sentence": "Molecular identification through whole genome sequencing indicated that the bacteria isolated from human breast milk were identified as Bifidobacterium breve (BS2-PS1 and BS2-PS2) and Lacticaseibacillus paracasei subspecies paracasei (BS2-PB5), whereas the bacteria isolated from infant feces was identified as Lactiplantibacillus plantarum (BR-12).",
                            "source": "openalex",
                            "title": "Whole-Genome Analysis and Tolerance Assessment on Bifidobacteria-like Bacteria Isolated from Breast Milk and Infant Fecal Samples in Indonesia",
                            "url": "https://doi.org/10.22146/jtbb.15062",
                            "year": "2025",
                            "id": "https://openalex.org/W4412096136"
                        },
                        {
                            "sentence": "Whole genome sequencing has played a significant role in the identification and characterization of many probiotic candidates.",
                            "source": "openalex",
                            "title": "Unveiling the probiotic potential of L. rhamnosus strain 044AE by genomic and phenotypic characterization",
                            "url": "https://doi.org/10.3934/microbiol.2026007",
                            "year": "2026",
                            "id": "https://openalex.org/W7151940960"
                        }
                    ],
                    "inferred": false
                },
                {
                    "key": "starch_hydrolysis",
                    "value": "Starch hydrolysis",
                    "score": 8.28,
                    "sources": [
                        "europepmc",
                        "crossref",
                        "openalex",
                        "doaj"
                    ],
                    "n_sources": 4,
                    "confidence": "moderate",
                    "evidence": [
                        {
                            "sentence": "Furthermore, compared to NC, L. chiayiensis AACE3, the activity of intestinal digestive enzymes (i.e., α-amylase, lipase and trypsin) was increased.",
                            "source": "europepmc",
                            "title": "Effects of Dietary Supplementation of <i>Lacticaseibacillus Chiayiensis</i> AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao Chicks.",
                            "url": "https://doi.org/10.3390/antibiotics12091356",
                            "year": "2023",
                            "id": "MED:37760653 doi:10.3390/antibiotics12091356"
                        },
                        {
                            "sentence": "Furthermore, compared to NC, L. chiayiensis AACE3, the activity of intestinal digestive enzymes (i.e., α-amylase, lipase and trypsin) was increased.",
                            "source": "crossref",
                            "title": "Effects of Dietary Supplementation of Lacticaseibacillus Chiayiensis AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao Chicks",
                            "url": "https://doi.org/10.3390/antibiotics12091356",
                            "year": "2023",
                            "id": "doi:10.3390/antibiotics12091356"
                        },
                        {
                            "sentence": "Furthermore, compared to NC, L. chiayiensis AACE3, the activity of intestinal digestive enzymes (i.e., α-amylase, lipase and trypsin) was increased.",
                            "source": "openalex",
                            "title": "Effects of Dietary Supplementation of Lacticaseibacillus Chiayiensis AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao Chicks",
                            "url": "https://doi.org/10.3390/antibiotics12091356",
                            "year": "2023",
                            "id": "https://openalex.org/W4386091526"
                        },
                        {
                            "sentence": "Furthermore, compared to NC, L. chiayiensis AACE3, the activity of intestinal digestive enzymes (i.e., α-amylase, lipase and trypsin) was increased.",
                            "source": "doaj",
                            "title": "Effects of Dietary Supplementation of Lacticaseibacillus Chiayiensis AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao Chicks",
                            "url": "https://www.mdpi.com/2079-6382/12/9/1356",
                            "year": "2023",
                            "id": "ae1254e5b1bd4b52b70914ab3f055dc8"
                        }
                    ],
                    "inferred": false
                },
                {
                    "key": "lipase",
                    "value": "Lipase",
                    "score": 8.28,
                    "sources": [
                        "europepmc",
                        "crossref",
                        "openalex",
                        "doaj"
                    ],
                    "n_sources": 4,
                    "confidence": "moderate",
                    "evidence": [
                        {
                            "sentence": "Furthermore, compared to NC, L. chiayiensis AACE3, the activity of intestinal digestive enzymes (i.e., α-amylase, lipase and trypsin) was increased.",
                            "source": "europepmc",
                            "title": "Effects of Dietary Supplementation of <i>Lacticaseibacillus Chiayiensis</i> AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao Chicks.",
                            "url": "https://doi.org/10.3390/antibiotics12091356",
                            "year": "2023",
                            "id": "MED:37760653 doi:10.3390/antibiotics12091356"
                        },
                        {
                            "sentence": "Furthermore, compared to NC, L. chiayiensis AACE3, the activity of intestinal digestive enzymes (i.e., α-amylase, lipase and trypsin) was increased.",
                            "source": "crossref",
                            "title": "Effects of Dietary Supplementation of Lacticaseibacillus Chiayiensis AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao Chicks",
                            "url": "https://doi.org/10.3390/antibiotics12091356",
                            "year": "2023",
                            "id": "doi:10.3390/antibiotics12091356"
                        },
                        {
                            "sentence": "Furthermore, compared to NC, L. chiayiensis AACE3, the activity of intestinal digestive enzymes (i.e., α-amylase, lipase and trypsin) was increased.",
                            "source": "openalex",
                            "title": "Effects of Dietary Supplementation of Lacticaseibacillus Chiayiensis AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao Chicks",
                            "url": "https://doi.org/10.3390/antibiotics12091356",
                            "year": "2023",
                            "id": "https://openalex.org/W4386091526"
                        },
                        {
                            "sentence": "Furthermore, compared to NC, L. chiayiensis AACE3, the activity of intestinal digestive enzymes (i.e., α-amylase, lipase and trypsin) was increased.",
                            "source": "doaj",
                            "title": "Effects of Dietary Supplementation of Lacticaseibacillus Chiayiensis AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao Chicks",
                            "url": "https://www.mdpi.com/2079-6382/12/9/1356",
                            "year": "2023",
                            "id": "ae1254e5b1bd4b52b70914ab3f055dc8"
                        }
                    ],
                    "inferred": false
                },
                {
                    "key": "haemolysis_pattern",
                    "value": "Haemolysis pattern",
                    "score": 4.66,
                    "sources": [
                        "openalex",
                        "doaj"
                    ],
                    "n_sources": 2,
                    "confidence": "moderate",
                    "evidence": [
                        {
                            "sentence": "L. paracasei TISTR 2593 exhibited γ-hemolytic activity (nonhemolysis) and demonstrated susceptibility to antibiotics, indicating that it is generally safe for consumption.",
                            "source": "openalex",
                            "title": "Evaluating the safety and efficacy of Lacticaseibacillus paracasei TISTR 2593 as a therapeutic probiotic for obesity prevention",
                            "url": "https://doi.org/10.3389/fmicb.2025.1501395",
                            "year": "2025",
                            "id": "https://openalex.org/W4409074428"
                        },
                        {
                            "sentence": "Regarding safety, the strain was susceptible to most clinically relevant antibiotics, such as tetracycline and ampicillin, and it did not exhibit hemolytic or gelatinase activity.",
                            "source": "openalex",
                            "title": "In vitro evaluation of Zymomonas mobilis UFPEDA 363 as a probiotic: safety profile, antagonism, and simulated gastrointestinal resistance",
                            "url": "https://doi.org/10.1590/0001-3765202620250788",
                            "year": "2026",
                            "id": "https://openalex.org/W7169801540"
                        },
                        {
                            "sentence": "Rigorous in vitro experiments confirmed tolerance of strain cqf-43 in the intestinal environment, its antimicrobial efficacy, sensitivity to antibiotics, absence of hemolysis and gelatinase activity, and its inability to produce biogenic amines.",
                            "source": "openalex",
                            "title": "Probiotic Potential and Safety Assessment of Lactiplantibacillus plantarum cqf-43 and Whole-Genome Sequence Analysis",
                            "url": "https://doi.org/10.3390/ijms242417570",
                            "year": "2023",
                            "id": "https://openalex.org/W4389894774"
                        },
                        {
                            "sentence": "In vitro experiments indicated that the Lpb. plantarum strain Y300 had no hemolytic activity; excellent acid-producing ability;an outstanding tolerance to bile salts, low-pH environments, and simulated gastrointestinal fluids; a positive hydrophobic interaction with xylene, and good auto-aggregation characteristics.",
                            "source": "openalex",
                            "title": "Complete Genome Sequence Analysis and Probiotic Characterisation of Lactiplantibacillus plantarum Y300 Isolated from Traditional Free-Range Chickens",
                            "url": "https://doi.org/10.3390/microorganisms13122738",
                            "year": "2025",
                            "id": "https://openalex.org/W4416916710"
                        },
                        {
                            "sentence": "In addition, PD2 demonstrated notable auto- and co-aggregation abilities with foodborne pathogens including Bacillus cereus, Escherichia coli, Enterococcus faecalis, Listeria monocytogenes, Salmonella Typhi, Vibrio cholerae, and V. parahaemolyticus, along with significant antioxidant activity and broad-spectrum antibacterial effects against those pathogens.",
                            "source": "doaj",
                            "title": "Genomic insights into Lacticaseibacillus chiayiensis PD2 isolated from Southern Thai fermented fish and its potential for probiotic and antibacterial activity against foodborne pathogens",
                            "url": "http://www.sciencedirect.com/science/article/pii/S2666154325006386",
                            "year": "2025",
                            "id": "2b6af0542f284517b45798f975d4edc9"
                        }
                    ],
                    "inferred": false
                },
                {
                    "key": "antibiotic_susceptibility_profile",
                    "value": "Antibiotic susceptibility profile",
                    "score": 4.27,
                    "sources": [
                        "pubmed",
                        "pubmed_pheno",
                        "europepmc",
                        "openalex"
                    ],
                    "n_sources": 4,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "These strains also demonstrated strong bile salt hydrolase (BSH) activity, high tolerance to simulated gastrointestinal conditions, resistance to phenol, broad-spectrum antimicrobial activity against foodborne pathogens, and favorable antibiotic susceptibility profiles.",
                            "source": "pubmed",
                            "title": "Multifunctional biotherapeutic probiotic bacteria with antioxidant capacity for cholesterol reduction, and inflammation alleviation.",
                            "url": "https://pubmed.ncbi.nlm.nih.gov/42008077/",
                            "year": "2026",
                            "id": "PMID:42008077"
                        },
                        {
                            "sentence": "These strains also demonstrated strong bile salt hydrolase (BSH) activity, high tolerance to simulated gastrointestinal conditions, resistance to phenol, broad-spectrum antimicrobial activity against foodborne pathogens, and favorable antibiotic susceptibility profiles.",
                            "source": "pubmed_pheno",
                            "title": "Multifunctional biotherapeutic probiotic bacteria with antioxidant capacity for cholesterol reduction, and inflammation alleviation.",
                            "url": "https://pubmed.ncbi.nlm.nih.gov/42008077/",
                            "year": "2026",
                            "id": "PMID:42008077"
                        },
                        {
                            "sentence": "These strains also demonstrated strong bile salt hydrolase (BSH) activity, high tolerance to simulated gastrointestinal conditions, resistance to phenol, broad-spectrum antimicrobial activity against foodborne pathogens, and favorable antibiotic susceptibility profiles.",
                            "source": "europepmc",
                            "title": "Multifunctional biotherapeutic probiotic bacteria with antioxidant capacity for cholesterol reduction, and inflammation alleviation.",
                            "url": "https://doi.org/10.1007/s12223-026-01477-3",
                            "year": "2026",
                            "id": "MED:42008077 doi:10.1007/s12223-026-01477-3"
                        },
                        {
                            "sentence": "We identified two Brevundimonas nasdae strains, Au-Bre29 and Au-Bre30, among these eight strains, having a high minimum inhibitory concentration (MIC) for As(III).",
                            "source": "openalex",
                            "title": "Characterization of Two Highly Arsenic-Resistant Caulobacteraceae Strains of Brevundimonas nasdae: Discovery of a New Arsenic Resistance Determinant",
                            "url": "https://doi.org/10.3390/ijms23105619",
                            "year": "2022",
                            "id": "https://openalex.org/W4280514026"
                        }
                    ],
                    "inferred": false
                },
                {
                    "key": "gelatin_hydrolysis",
                    "value": "Gelatin hydrolysis — reported negative",
                    "score": 2.79,
                    "sources": [
                        "openalex"
                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "Regarding safety, the strain was susceptible to most clinically relevant antibiotics, such as tetracycline and ampicillin, and it did not exhibit hemolytic or gelatinase activity.",
                            "source": "openalex",
                            "title": "In vitro evaluation of Zymomonas mobilis UFPEDA 363 as a probiotic: safety profile, antagonism, and simulated gastrointestinal resistance",
                            "url": "https://doi.org/10.1590/0001-3765202620250788",
                            "year": "2026",
                            "id": "https://openalex.org/W7169801540"
                        },
                        {
                            "sentence": "Furthermore, the genome is devoid of menacing genes such as hemolysin, gelatinase, and biogenic amine-producing genes.",
                            "source": "openalex",
                            "title": "Probiotic Potential and Safety Assessment of Lactiplantibacillus plantarum cqf-43 and Whole-Genome Sequence Analysis",
                            "url": "https://doi.org/10.3390/ijms242417570",
                            "year": "2023",
                            "id": "https://openalex.org/W4389894774"
                        },
                        {
                            "sentence": "Rigorous in vitro experiments confirmed tolerance of strain cqf-43 in the intestinal environment, its antimicrobial efficacy, sensitivity to antibiotics, absence of hemolysis and gelatinase activity, and its inability to produce biogenic amines.",
                            "source": "openalex",
                            "title": "Probiotic Potential and Safety Assessment of Lactiplantibacillus plantarum cqf-43 and Whole-Genome Sequence Analysis",
                            "url": "https://doi.org/10.3390/ijms242417570",
                            "year": "2023",
                            "id": "https://openalex.org/W4389894774"
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                    ],
                    "inferred": false
                },
                {
                    "key": "motility",
                    "value": "Motility — reported negative",
                    "score": 0.93,
                    "sources": [
                        "europepmc"
                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "Four rod-shaped, non-motile, non-spore-forming, facultative anaerobic, Gram-stain-positive lactic acid bacteria, designated as EB0058 T , SCR0080, LD0937 T and SCR0063 T , were isolated from different corn and grass silage samples.",
                            "source": "europepmc",
                            "title": "&lt;i&gt;Lacticaseibacillus parahuelsenbergensis&lt;/i&gt; sp. nov., &lt;i&gt;Lacticaseibacillus styriensis&lt;/i&gt; sp. nov. and &lt;i&gt;Lacticaseibacillus zeae&lt;/i&gt; subsp. &lt;i&gt;silagei&lt;/i&gt; subsp. nov., isolated from different grass and corn silage.",
                            "url": "https://doi.org/10.1099/ijsem.0.006441",
                            "year": "2024",
                            "id": "MED:38954457 doi:10.1099/ijsem.0.006441"
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                    ],
                    "inferred": false
                },
                {
                    "key": "spore_formation_stain",
                    "value": "Spore formation / stain — reported negative",
                    "score": 0.93,
                    "sources": [
                        "europepmc"
                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "Four rod-shaped, non-motile, non-spore-forming, facultative anaerobic, Gram-stain-positive lactic acid bacteria, designated as EB0058 T , SCR0080, LD0937 T and SCR0063 T , were isolated from different corn and grass silage samples.",
                            "source": "europepmc",
                            "title": "&lt;i&gt;Lacticaseibacillus parahuelsenbergensis&lt;/i&gt; sp. nov., &lt;i&gt;Lacticaseibacillus styriensis&lt;/i&gt; sp. nov. and &lt;i&gt;Lacticaseibacillus zeae&lt;/i&gt; subsp. &lt;i&gt;silagei&lt;/i&gt; subsp. nov., isolated from different grass and corn silage.",
                            "url": "https://doi.org/10.1099/ijsem.0.006441",
                            "year": "2024",
                            "id": "MED:38954457 doi:10.1099/ijsem.0.006441"
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                    ],
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                },
                {
                    "key": "aerotolerance_test",
                    "value": "Aerotolerance test — reported positive",
                    "score": 0.93,
                    "sources": [
                        "europepmc"
                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "Four rod-shaped, non-motile, non-spore-forming, facultative anaerobic, Gram-stain-positive lactic acid bacteria, designated as EB0058 T , SCR0080, LD0937 T and SCR0063 T , were isolated from different corn and grass silage samples.",
                            "source": "europepmc",
                            "title": "&lt;i&gt;Lacticaseibacillus parahuelsenbergensis&lt;/i&gt; sp. nov., &lt;i&gt;Lacticaseibacillus styriensis&lt;/i&gt; sp. nov. and &lt;i&gt;Lacticaseibacillus zeae&lt;/i&gt; subsp. &lt;i&gt;silagei&lt;/i&gt; subsp. nov., isolated from different grass and corn silage.",
                            "url": "https://doi.org/10.1099/ijsem.0.006441",
                            "year": "2024",
                            "id": "MED:38954457 doi:10.1099/ijsem.0.006441"
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                    ],
                    "inferred": false
                },
                {
                    "key": "short_chain_fatty_acid_profile_glc_",
                    "value": "Short-chain fatty acid profile (GLC)",
                    "score": 0.93,
                    "sources": [
                        "europepmc"
                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "Probiotics produce the secondary metabolites, such as short-chain fatty acids, hydrogen peroxide, enzymes, and bacteriocins, that inhibit the proliferation of other resident gut microbes.",
                            "source": "europepmc",
                            "title": "Mechanisms of probiotic action in poultry gut: Colonization resistance, bioactive metabolites, quorum sensing activity, and immunomodulation.",
                            "url": "https://doi.org/10.1016/j.psj.2026.107616",
                            "year": "2026",
                            "id": "MED:42736181 doi:10.1016/j.psj.2026.107616"
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                    ],
                    "inferred": false
                },
                {
                    "key": "carbohydrate_fermentation_panel",
                    "value": "Carbohydrate fermentation panel",
                    "score": 0.93,
                    "sources": [
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                    ],
                    "n_sources": 1,
                    "confidence": "low",
                    "evidence": [
                        {
                            "sentence": "Functional annotations derived from COG and KEGG databases highlighted a significant abundance of genes related to metabolism, with a notable emphasis on carbohydrate utilization.",
                            "source": "openalex",
                            "title": "Probiotic Potential and Safety Assessment of Lactiplantibacillus plantarum cqf-43 and Whole-Genome Sequence Analysis",
                            "url": "https://doi.org/10.3390/ijms242417570",
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                            "id": "https://openalex.org/W4389894774"
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                    ],
                    "inferred": false
                }
            ],
            "confidence": "high",
            "conflict": null,
            "review": "unreviewed"
        }
    },
    "references": [
        {
            "title": "Genomic and in-vitro characteristics of a novel strain Lacticaseibacillus chiayiensis AACE3 isolated from fermented blueberry",
            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10235500/",
            "year": "2023",
            "id": "PMC10235500",
            "source": "pmc",
            "fields": [
                "media",
                "time",
                "gram",
                "temperature"
            ],
            "n": 12
        },
        {
            "title": "Clinical Potential of Novel Microbial Therapeutic LP51 Based on Xerosis-Microbiome Index",
            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11639849/",
            "year": "2024",
            "id": "PMC11639849",
            "source": "pmc",
            "fields": [
                "media",
                "time",
                "biochem"
            ],
            "n": 6
        },
        {
            "title": "Effects of Dietary Supplementation of <i>Lacticaseibacillus Chiayiensis</i> AACE3 on Hepatic Antioxidant Capacity, Immune Factors and Gut Microbiology in Nandan Yao Chicks.",
            "url": "https://doi.org/10.3390/antibiotics12091356",
            "year": "2023",
            "id": "MED:37760653 doi:10.3390/antibiotics12091356",
            "source": "europepmc",
            "fields": [
                "biochem"
            ],
            "n": 6
        },
        {
            "title": "&lt;i&gt;Lacticaseibacillus parahuelsenbergensis&lt;/i&gt; sp. nov., &lt;i&gt;Lacticaseibacillus styriensis&lt;/i&gt; sp. nov. and &lt;i&gt;Lacticaseibacillus zeae&lt;/i&gt; subsp. &lt;i&gt;silagei&lt;/i&gt; subsp. nov., isolated from different grass and corn silage.",
            "url": "https://doi.org/10.1099/ijsem.0.006441",
            "year": "2024",
            "id": "MED:38954457 doi:10.1099/ijsem.0.006441",
            "source": "europepmc",
            "fields": [
                "gram",
                "biochem"
            ],
            "n": 6
        },
        {
            "title": "Whole-Genome Sequence Analysis, Probiotic Potential, and Safety Assessment of the Marine Bacterium Paraliobacillus zengyii CGMCC1.16464",
            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12112907/",
            "year": "2025",
            "id": "PMC12112907",
            "source": "pmc",
            "fields": [
                "media",
                "temperature",
                "time"
            ],
            "n": 5
        },
        {
            "title": "Complete Genome Sequencing and Comparative Genomics of Three Potential Probiotic Strains, Lacticaseibacillus casei FBL6, Lacticaseibacillus chiayiensis FBL7, and Lacticaseibacillus zeae FBL8",
            "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777060/",
            "year": "2022",
            "id": "PMC8777060",
            "source": "pmc",
            "fields": [
                "media",
                "time",
                "biochem"
            ],
            "n": 4
        },
        {
            "title": "Probiotic Potential and Safety Assessment of Lactiplantibacillus plantarum cqf-43 and Whole-Genome Sequence Analysis",
            "url": "https://doi.org/10.3390/ijms242417570",
            "year": "2023",
            "id": "https://openalex.org/W4389894774",
            "source": "openalex",
            "fields": [
                "biochem"
            ],
            "n": 4
        },
        {
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