{ "organism": "Staphylococcus agnetis", "built_at": "2026-08-12T21:25:53+05:30", "review_status": "unreviewed", "generator": "CultureScope 1.0.0", "disclaimer": "Aggregated from published literature. Unreviewed. Not a substitute for a validated SOP or an accredited reference laboratory.", "taxonomy": { "taxon": "Staphylococcus agnetis", "lineage": "cellular organisms; Bacteria; Bacillati; Bacillota; Bacilli; Caryophanales; Staphylococcaceae; Staphylococcus", "rank": "species", "taxid": "985762", "taxonomy_url": "https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=985762", "gram_prior": { "value": "Gram-positive", "basis": "Inferred from NCBI Taxonomy lineage" }, "accepted": "Staphylococcus agnetis", "gbif_status": "ACCEPTED", "gbif_name": "Staphylococcus agnetis Taponen et al., 2012" }, "fields": { "media": { "label": "Media used", "multi": true, "items": [ { "key": "brain_heart_infusion_bhi_", "value": "Brain heart infusion (BHI)", "score": 10.8, "sources": [ "pubmed", "crossref", "openalex", "doaj" ], "n_sources": 4, "confidence": "high", "evidence": [ { "sentence": "The inhibitory activity of S. agnetis 3682 against 20 multidrug-resistant MRSA strains involved in human infections in two Brazilian hospitals was determined by the deferred antagonism assay on brain-heart infusion (BHI) agar plates.", "source": "pubmed", "title": "Draft genome sequence of Staphylococcus agnetis 3682, the producing strain of the broad-spectrum lantibiotic agneticin 3682.", "url": "https://pubmed.ncbi.nlm.nih.gov/31454564/", "year": "2019", "id": "PMID:31454564" }, { "sentence": "Investigation of the spectrum of activity of S. agnetis 4244 was performed on BHI agar by deferred antagonism assay.", "source": "pubmed", "title": "Draft genome sequence of Staphylococcus agnetis 4244, a strain with gene clusters encoding distinct post-translationally modified antimicrobial peptides.", "url": "https://pubmed.ncbi.nlm.nih.gov/34700053/", "year": "2021", "id": "PMID:34700053" }, { "sentence": "Here, we reconstituted a skin model using keratin as a base (instead of callus) and compared it with brain heart infusion (BHI) medium.", "source": "crossref", "title": "Effects of cosmetic ingredients on growth and virulence factor expression in Staphylococcus aureus: a comparison between culture medium and in vitro skin model medium", "url": "https://doi.org/10.3934/microbiol.2025002", "year": "2024", "id": "doi:10.3934/microbiol.2025002" }, { "sentence": "Upon the addition of cosmetic ingredients to BHI or skin model medium, the sensitivity of S. aureus to these cosmetic ingredients differed between the two media.", "source": "crossref", "title": "Effects of cosmetic ingredients on growth and virulence factor expression in Staphylococcus aureus: a comparison between culture medium and in vitro skin model medium", "url": "https://doi.org/10.3934/microbiol.2025002", "year": "2024", "id": "doi:10.3934/microbiol.2025002" }, { "sentence": "Notably, after adding the two tested cosmetic ingredients, the expression level of staphylococcal enterotoxin A in S. aureus reduced significantly in skin model medium compared with that in the BHI medium.", "source": "crossref", "title": "Effects of cosmetic ingredients on growth and virulence factor expression in Staphylococcus aureus: a comparison between culture medium and in vitro skin model medium", "url": "https://doi.org/10.3934/microbiol.2025002", "year": "2024", "id": "doi:10.3934/microbiol.2025002" }, { "sentence": "Investigation of the spectrum of activity of S. agnetis 4244 was performed on BHI agar by deferred antagonism assay.", "source": "openalex", "title": "Draft genome sequence of Staphylococcus agnetis 4244, a strain with gene clusters encoding distinct post-translationally modified antimicrobial peptides", "url": "https://doi.org/10.1016/j.jgar.2021.10.006", "year": "2021", "id": "https://openalex.org/W3207983804" } ], "inferred": false }, { "key": "blood_agar", "value": "Blood agar", "score": 7.15, "sources": [ "pmc", "europepmc" ], "n_sources": 2, "confidence": "moderate", "evidence": [ { "sentence": "All lesion swabs produced small, round, white, nonhemolytic, Gram-positive staphylococcus -like colonies on blood agar.", "source": "pmc", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430782/", "year": "2022", "id": "PMC9430782" }, { "sentence": "Eight lesion swabs collected from H4 also yielded staphylococcal growth in blood agar, and the bacteria were isolated as pure cultures from three swabs.", "source": "pmc", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430782/", "year": "2022", "id": "PMC9430782" }, { "sentence": "Each swab was used to streak 5% (vol/vol) sheep blood agar and MacConkey agar within 24 h of collection.", "source": "pmc", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430782/", "year": "2022", "id": "PMC9430782" }, { "sentence": "Briefly, five BFs from each animal were washed three times by dipping into 250 μL of sterile normal saline, and the saline washing solution from each group was streaked onto blood agar and MacConkey agar.", "source": "pmc", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430782/", "year": "2022", "id": "PMC9430782" }, { "sentence": "Flies were then rinsed in normal saline, air dried, homogenized in 100 μL of sterile normal saline, and plated on blood agar and MacConkey agar plates.", "source": "pmc", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430782/", "year": "2022", "id": "PMC9430782" }, { "sentence": "The objective of this prospective study was a blinded comparison of three methods for the identification of bacteria isolated on Columbia blood agar from milk samples of dairy cows.", "source": "europepmc", "title": "Test Agreement among Biochemical Methods, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, and 16S rRNA Sequencing for Identification of Microorganisms Isolated from Bovine Milk.", "url": "https://doi.org/10.1128/jcm.01381-18", "year": "2019", "id": "MED:30567749 doi:10.1128/jcm.01381-18" } ], "inferred": false }, { "key": "tryptic_soy_broth_tsb_", "value": "Tryptic soy broth (TSB)", "score": 5.73, "sources": [ "pmc", "openalex" ], "n_sources": 2, "confidence": "moderate", "evidence": [ { "sentence": "Media included: CHROMagar Orientation (DRG International, Springfield Township, NJ), tryptic soy broth (Difco brand, Becton, Dickinson and Company, Franklin Lakes, NJ); and Luria broth (LB; per liter 10 g tryptone, 5 g yeast extract, 5 g NaCl).", "source": "pmc", "title": "Embryo lethality assay as a tool for assessing virulence of isolates from bacterial chondronecrosis with osteomyelitis in broilers", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496174/", "year": "2021", "id": "PMC8496174" }, { "sentence": "De novo transcriptome analysis of halotolerant bacterium Staphylococcus sp. strain P-TSB-70 isolated from East coast of India: In search of salt stress tolerant genes.", "source": "openalex", "title": "De novo transcriptome analysis of halotolerant bacterium Staphylococcus sp. strain P-TSB-70 isolated from East coast of India: In search of salt stress tolerant genes", "url": "https://doi.org/10.1371/journal.pone.0228199", "year": "2020", "id": "https://openalex.org/W3005545504" }, { "sentence": "In favour of whole-genome transcriptome profiling of Staphylococcus sp. strain P-TSB-70 (GenBank Accn. no. KP117091) which tolerated upto 20% NaCl stress, the strain was cultured in the laboratory condition with 20% NaCl stress.", "source": "openalex", "title": "De novo transcriptome analysis of halotolerant bacterium Staphylococcus sp. strain P-TSB-70 isolated from East coast of India: In search of salt stress tolerant genes", "url": "https://doi.org/10.1371/journal.pone.0228199", "year": "2020", "id": "https://openalex.org/W3005545504" } ], "inferred": false }, { "key": "macconkey_agar", "value": "MacConkey agar", "score": 3.73, "sources": [ "pmc" ], "n_sources": 1, "confidence": "low", "evidence": [ { "sentence": "All the 2020 and 2021 isolates were reidentified by PCR, and no bacterial growth was observed following plating on MacConkey agar.", "source": "pmc", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430782/", "year": "2022", "id": "PMC9430782" }, { "sentence": "Each swab was used to streak 5% (vol/vol) sheep blood agar and MacConkey agar within 24 h of collection.", "source": "pmc", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430782/", "year": "2022", "id": "PMC9430782" }, { "sentence": "Briefly, five BFs from each animal were washed three times by dipping into 250 μL of sterile normal saline, and the saline washing solution from each group was streaked onto blood agar and MacConkey agar.", "source": "pmc", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430782/", "year": "2022", "id": "PMC9430782" }, { "sentence": "Flies were then rinsed in normal saline, air dried, homogenized in 100 μL of sterile normal saline, and plated on blood agar and MacConkey agar plates.", "source": "pmc", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430782/", "year": "2022", "id": "PMC9430782" } ], "inferred": false }, { "key": "mannitol_salt_agar", "value": "Mannitol salt agar", "score": 1.35, "sources": [ "pubmed" ], "n_sources": 1, "confidence": "low", "evidence": [ { "sentence": "Swabbing samples were mixed with 10 mL of sterile saline, agitated, and cultured on mannitol salt agar plates.", "source": "pubmed", "title": "Cross-sectional study to identify staphylococcal species isolated from teat and inguinal skin of different-aged dairy heifers.", "url": "https://pubmed.ncbi.nlm.nih.gov/29397170/", "year": "2018", "id": "PMID:29397170" } ], "inferred": false }, { "key": "chromogenic_uti_orientation", "value": "Chromogenic UTI / Orientation", "score": 0.93, "sources": [ "pmc" ], "n_sources": 1, "confidence": "low", "evidence": [ { "sentence": "Media included: CHROMagar Orientation (DRG International, Springfield Township, NJ), tryptic soy broth (Difco brand, Becton, Dickinson and Company, Franklin Lakes, NJ); and Luria broth (LB; per liter 10 g tryptone, 5 g yeast extract, 5 g NaCl).", "source": "pmc", "title": "Embryo lethality assay as a tool for assessing virulence of isolates from bacterial chondronecrosis with osteomyelitis in broilers", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496174/", "year": "2021", "id": "PMC8496174" } ], "inferred": false } ], "confidence": "high", "conflict": null, "review": "unreviewed" }, "temperature": { "label": "Optimum temperature for growth", "multi": false, "items": [], "confidence": "none", "conflict": null, "review": "unreviewed" }, "time": { "label": "Time required to grow", "multi": false, "items": [ { "key": "hrs_24_24", "value": "24 h", "score": 3.62, "sources": [ "pubmed", "pmc", "openalex" ], "n_sources": 3, "confidence": "low", "evidence": [ { "sentence": "At 24 h, plates were read and up to 10 staphylococcal colonies were saved for further analysis.", "source": "pubmed", "title": "Cross-sectional study to identify staphylococcal species isolated from teat and inguinal skin of different-aged dairy heifers.", "url": "https://pubmed.ncbi.nlm.nih.gov/29397170/", "year": "2018", "id": "PMID:29397170" }, { "sentence": "Each swab was used to streak 5% (vol/vol) sheep blood agar and MacConkey agar within 24 h of collection.", "source": "pmc", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430782/", "year": "2022", "id": "PMC9430782" }, { "sentence": "Plates were incubated at 37 degC for 24 h.", "source": "pmc", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430782/", "year": "2022", "id": "PMC9430782" }, { "sentence": "One of these isolates, identified as Staph. epidermidis , no. 605, exhibited the highest activity compared to others, degrading available histamine to 58.33% within 24 h.", "source": "openalex", "title": "Inhibition of histamine accumulation by novel histamine‐degrading species of Staphylococcus sp. isolated from goats and sheep milk", "url": "https://doi.org/10.1002/fsn3.2723", "year": "2022", "id": "https://openalex.org/W4206185474" } ], "inferred": false } ], "confidence": "low", "conflict": null, "review": "unreviewed" }, "colony": { "label": "Colony morphology", "multi": false, "items": [ { "key": "c675a6ea632", "value": "All lesion swabs produced small, round, white, nonhemolytic, Gram-positive staphylococcus -like colonies on blood agar.", "score": 2.42, "sources": [ "pmc" ], "n_sources": 1, "confidence": "low", "evidence": [ { "sentence": "All lesion swabs produced small, round, white, nonhemolytic, Gram-positive staphylococcus -like colonies on blood agar.", "source": "pmc", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430782/", "year": "2022", "id": "PMC9430782" } ], "inferred": false }, { "key": "cb2a789a04a", "value": "A normal skin swab from one H4 animal produced only one colony of Staphylococcus sp., while BF washings from one animal and homogenized BFs from two animals yielded two staphylococcus -like colonies and heavy staphylococcus -like growth, respectively.", "score": 2.42, "sources": [ "pmc" ], "n_sources": 1, "confidence": "low", "evidence": [ { "sentence": "A normal skin swab from one H4 animal produced only one colony of Staphylococcus sp., while BF washings from one animal and homogenized BFs from two animals yielded two staphylococcus -like colonies and heavy staphylococcus -like growth, respectively.", "source": "pmc", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430782/", "year": "2022", "id": "PMC9430782" } ], "inferred": false } ], "confidence": "low", "conflict": null, "review": "unreviewed" }, "gram": { "label": "Gram character", "multi": false, "items": [ { "key": "gram_positive", "value": "Gram-positive", "score": 43.73, "sources": [ "pubmed", "pubmed_pheno", "pmc", "europepmc", "openalex", "doaj", "wikipedia" ], "n_sources": 7, "confidence": "high", "evidence": [ { "sentence": "Thirteen Gram-positive-staining coagulase-variable staphylococci were isolated from subclinical and mild clinical mastitic bovine milk (n=12) and a teat apex (n=1).", "source": "pubmed", "title": "Staphylococcus agnetis sp. nov., a coagulase-variable species from bovine subclinical and mild clinical mastitis.", "url": "https://pubmed.ncbi.nlm.nih.gov/21335502/", "year": "2012", "id": "PMID:21335502" }, { "sentence": "Here we report the draft genome sequence of Staphylococcus agnetis 4244, a strain involved in bovine mastitis, and its ability to inhibit different species of antibiotic-resistant Gram-positive bacteria owing to bacteriocin production.", "source": "pubmed", "title": "Draft genome sequence of Staphylococcus agnetis 4244, a strain with gene clusters encoding distinct post-translationally modified antimicrobial peptides.", "url": "https://pubmed.ncbi.nlm.nih.gov/34700053/", "year": "2021", "id": "PMID:34700053" }, { "sentence": "Staphylococcus agnetis 4244 was able to inhibit all 44 strains of antibiotic-resistant Gram-positive bacteria tested in this study, including vancomycin-resistant enterococci (VRE), methicillin-resistant Staphylococcus aureus (MRSA) and other antibiotic-resistant staphylococcal strains.", "source": "pubmed", "title": "Draft genome sequence of Staphylococcus agnetis 4244, a strain with gene clusters encoding distinct post-translationally modified antimicrobial peptides.", "url": "https://pubmed.ncbi.nlm.nih.gov/34700053/", "year": "2021", "id": "PMID:34700053" }, { "sentence": "This study emphasises the potential biotechnological application of this strain for production of bacteriocins that could be used in the food industry as biopreservatives and/or in medicine as alternative therapeutic options against VRE, MRSA, vancomycin-intermediate S. aureus and other antibiotic-resistant Gram-positive bacteria, including biofilm-forming isolates.", "source": "pubmed", "title": "Draft genome sequence of Staphylococcus agnetis 4244, a strain with gene clusters encoding distinct post-translationally modified antimicrobial peptides.", "url": "https://pubmed.ncbi.nlm.nih.gov/34700053/", "year": "2021", "id": "PMID:34700053" }, { "sentence": "The term BLIS is applied to uncharacterized proteinaceous antimicrobials produced by gram-positive bacteria.", "source": "pubmed", "title": "Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk.", "url": "https://pubmed.ncbi.nlm.nih.gov/30799112/", "year": "2019", "id": "PMID:30799112" }, { "sentence": "All the gram-positive targets, including Bacillus cereus, Listeria monocytogenes, Enterococcus faecalis Ef37 (a tyramine-producer strain), Lactobacillus saerimneri 30a (a histamine-producer strain), and methicillin-resistant Staph. epidermidis, were inhibited by the Staph. chromogenes isolates.", "source": "pubmed", "title": "Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk.", "url": "https://pubmed.ncbi.nlm.nih.gov/30799112/", "year": "2019", "id": "PMID:30799112" }, { "sentence": "Inferred from NCBI Taxonomy lineage: cellular organisms; Bacteria; Bacillati; Bacillota; Bacilli; Caryophanales; Staphylococcaceae; Staphylococcus", "source": "ncbi_taxonomy", "title": "NCBI Taxonomy lineage", "url": "https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=985762", "year": "2026", "id": "lineage" } ], "inferred": false }, { "key": "gram_negative", "value": "Gram-negative", "score": 1.89, "sources": [ "pubmed", "pubmed_pheno" ], "n_sources": 2, "confidence": "low", "evidence": [ { "sentence": "The antimicrobial compounds from test strains were not effective against gram-negative pathogens, including Salmonella enterica serovar Typhimurium, Escherichia coli O157:H7, and Klebsiella pneumonia as well as the indicator mold Aspergillus fumigatus.", "source": "pubmed", "title": "Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk.", "url": "https://pubmed.ncbi.nlm.nih.gov/30799112/", "year": "2019", "id": "PMID:30799112" }, { "sentence": "The antimicrobial compounds from test strains were not effective against gram-negative pathogens, including Salmonella enterica serovar Typhimurium, Escherichia coli O157:H7, and Klebsiella pneumonia as well as the indicator mold Aspergillus fumigatus.", "source": "pubmed_pheno", "title": "Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk.", "url": "https://pubmed.ncbi.nlm.nih.gov/30799112/", "year": "2019", "id": "PMID:30799112" } ], "inferred": false } ], "confidence": "high", "conflict": null, "review": "unreviewed" }, "biochem": { "label": "Biochemical tests for identification", "multi": true, "items": [ { "key": "coagulase", "value": "Coagulase", "score": 59.65, "sources": [ "pubmed", "pubmed_pheno", "europepmc", "openalex", "doaj", "wikipedia" ], "n_sources": 6, "confidence": "high", "evidence": [ { "sentence": "Staphylococcus hyicus and Staphylococcus agnetis are two coagulase-variable staphylococcal species that can be isolated from bovine milk and are difficult to differentiate.", "source": "pubmed", "title": "Species Identification and Strain Typing of Staphylococcus agnetis and Staphylococcus hyicus Isolates from Bovine Milk by Use of a Novel Multiplex PCR Assay and Pulsed-Field Gel Electrophoresis.", "url": "https://pubmed.ncbi.nlm.nih.gov/28330895/", "year": "2017", "id": "PMID:28330895" }, { "sentence": "The objectives of this study were to characterize isolates of bovine milk origin from a collection that had previously been characterized as coagulase-positive S. hyicus based on phenotypic species identification methods and to develop a PCR-based method for differentiating S. hyicus , S. agnetis , and Staphylococcus aureus Isolates ( n = 62) were selected from a previous study in which milk samples were collected from cows on 15 dairy herds.", "source": "pubmed", "title": "Species Identification and Strain Typing of Staphylococcus agnetis and Staphylococcus hyicus Isolates from Bovine Milk by Use of a Novel Multiplex PCR Assay and Pulsed-Field Gel Electrophoresis.", "url": "https://pubmed.ncbi.nlm.nih.gov/28330895/", "year": "2017", "id": "PMID:28330895" }, { "sentence": "Isolates were coagulase tested and identified to the species level using housekeeping gene sequencing.", "source": "pubmed", "title": "Species Identification and Strain Typing of Staphylococcus agnetis and Staphylococcus hyicus Isolates from Bovine Milk by Use of a Novel Multiplex PCR Assay and Pulsed-Field Gel Electrophoresis.", "url": "https://pubmed.ncbi.nlm.nih.gov/28330895/", "year": "2017", "id": "PMID:28330895" }, { "sentence": "Overall, 88% (37/42) of coagulase-positive S. agnetis isolates were found to be coagulase positive at 4 h.", "source": "pubmed", "title": "Species Identification and Strain Typing of Staphylococcus agnetis and Staphylococcus hyicus Isolates from Bovine Milk by Use of a Novel Multiplex PCR Assay and Pulsed-Field Gel Electrophoresis.", "url": "https://pubmed.ncbi.nlm.nih.gov/28330895/", "year": "2017", "id": "PMID:28330895" }, { "sentence": "The herd-level prevalence of coagulase-positive S. agnetis ranged from 0 to 2.17%.", "source": "pubmed", "title": "Species Identification and Strain Typing of Staphylococcus agnetis and Staphylococcus hyicus Isolates from Bovine Milk by Use of a Novel Multiplex PCR Assay and Pulsed-Field Gel Electrophoresis.", "url": "https://pubmed.ncbi.nlm.nih.gov/28330895/", "year": "2017", "id": "PMID:28330895" }, { "sentence": "Thirteen Gram-positive-staining coagulase-variable staphylococci were isolated from subclinical and mild clinical mastitic bovine milk (n=12) and a teat apex (n=1).", "source": "pubmed", "title": "Staphylococcus agnetis sp. nov., a coagulase-variable species from bovine subclinical and mild clinical mastitis.", "url": "https://pubmed.ncbi.nlm.nih.gov/21335502/", "year": "2012", "id": "PMID:21335502" } ], "inferred": false }, { "key": "maldi_tof_ms", "value": "MALDI-TOF MS", "score": 54.85, "sources": [ "pubmed", "pubmed_pheno", "pmc", "europepmc", "crossref", "openalex", "doaj", "preprints" ], "n_sources": 8, "confidence": "high", "evidence": [ { "sentence": "Bacterial identification was conducted by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) and strain typing by repetitive sequence-based PCR (rep-PCR) and DNA sequencing to determine species similarity and virulence factors.", "source": "pubmed", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle.", "url": "https://pubmed.ncbi.nlm.nih.gov/35863026/", "year": "2022", "id": "PMID:35863026" }, { "sentence": "The data was comprised of microbiological results from quarter CM samples routinely submitted to Quality Milk Production Services at Cornell University for microbial identification by MALDI-TOF mass spectrometry.", "source": "pubmed", "title": "Frequency of non-aureus staphylococci and mammaliicocci species isolated from quarter clinical mastitis: A 6-year retrospective study.", "url": "https://pubmed.ncbi.nlm.nih.gov/38056569/", "year": "2024", "id": "PMID:38056569" }, { "sentence": "The data was comprised of microbiological results from quarter CM samples routinely submitted to Quality Milk Production Services at Cornell University for microbial identification by MALDI-TOF mass spectrometry.", "source": "pubmed_pheno", "title": "Frequency of non-aureus staphylococci and mammaliicocci species isolated from quarter clinical mastitis: A 6-year retrospective study.", "url": "https://pubmed.ncbi.nlm.nih.gov/38056569/", "year": "2024", "id": "PMID:38056569" }, { "sentence": "Bacterial identification was conducted by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) and strain typing by repetitive sequence-based PCR (rep-PCR) and DNA sequencing to determine species similarity and virulence factors.", "source": "pubmed_pheno", "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle.", "url": "https://pubmed.ncbi.nlm.nih.gov/35863026/", "year": "2022", "id": "PMID:35863026" }, { "sentence": "Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR..", "source": "pubmed_pheno", "title": "Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR.", "url": "https://pubmed.ncbi.nlm.nih.gov/28532810/", "year": "2017", "id": "PMID:28532810" }, { "sentence": "Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples.", "source": "pubmed_pheno", "title": "Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR.", "url": "https://pubmed.ncbi.nlm.nih.gov/28532810/", "year": "2017", "id": "PMID:28532810" } ], "inferred": false }, { "key": "haemolysis_pattern", "value": "Haemolysis pattern", "score": 41.9, "sources": [ "pubmed", "pubmed_pheno", "pmc", "europepmc", "openalex", "doaj" ], "n_sources": 6, "confidence": "high", "evidence": [ { "sentence": "The most common species identified from body site samples were S. chromogenes, S. xylosus, and Staphylococcus haemolyticus.", "source": "pubmed", "title": "Molecular characterization of non-aureus Staphylococcus spp. from heifer intramammary infections and body sites.", "url": "https://pubmed.ncbi.nlm.nih.gov/29525303/", "year": "2018", "id": "PMID:29525303" }, { "sentence": "Overall, the most common species identified were Staphylococcus haemolyticus followed by Staphylococcus chromogenes, Staphylococcus xylosus, Staphylococcus devriesei, and Staphylococcus sciuri.", "source": "pubmed", "title": "Cross-sectional study to identify staphylococcal species isolated from teat and inguinal skin of different-aged dairy heifers.", "url": "https://pubmed.ncbi.nlm.nih.gov/29397170/", "year": "2018", "id": "PMID:29397170" }, { "sentence": "Staphylococcus haemolyticus 4S12 was able to inhibit the majority of gram-positive bacteria.", "source": "pubmed", "title": "Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk.", "url": "https://pubmed.ncbi.nlm.nih.gov/30799112/", "year": "2019", "id": "PMID:30799112" }, { "sentence": "Our results showed that 29 distinct NASM species were identified, with the 8 most prevalent NASM species being Staphylococcus chromogenes, Staphylococcus haemolyticus, Staphylococcus simulans, Staphylococcus epidermidis, Staphylococcus sciuri (now Mammaliicoccus sciuri), Staphylococcus agnetis/Staphylococcus hyicus, Staphylococcus borealis, and Staphylococcus xylosus.", "source": "pubmed", "title": "Frequency of non-aureus staphylococci and mammaliicocci species isolated from quarter clinical mastitis: A 6-year retrospective study.", "url": "https://pubmed.ncbi.nlm.nih.gov/38056569/", "year": "2024", "id": "PMID:38056569" }, { "sentence": "This study showed that Staphylococcus chromogenes remains the most frequent (43%) NASM species identified from bovine CM, followed by Staphylococcus haemolyticus (18%), and Staphylococcus simulans (12%).", "source": "pubmed", "title": "Frequency of non-aureus staphylococci and mammaliicocci species isolated from quarter clinical mastitis: A 6-year retrospective study.", "url": "https://pubmed.ncbi.nlm.nih.gov/38056569/", "year": "2024", "id": "PMID:38056569" }, { "sentence": "While two mecA -positive Staphylococcus epidermidis isolates from farm workers had SCC mec II and IV, a mecA -positive S. epidermidis isolate from broiler and a Staphylococcus haemolyticus isolate farm environment carried SCC mec V. The occurrence of multidrug resistance was observed in 48.1% (104/216 isolates) of NAS isolates with high resistance rates to β-lactams (>40%) and fusidic acid (59.7%).", "source": "pubmed", "title": "Species Profiles and Antimicrobial Resistance of Non- aureus Staphylococci Isolated from Healthy Broilers, Farm Environments, and Farm Workers.", "url": "https://pubmed.ncbi.nlm.nih.gov/37701746/", "year": "2023", "id": "PMID:37701746" } ], "inferred": false }, { "key": "16s_rrna_gene_sequencing", "value": "16S rRNA gene sequencing", "score": 15.1, "sources": [ "pubmed", "europepmc", "openalex" ], "n_sources": 3, "confidence": "high", "evidence": [ { "sentence": "Pathogens from bovine subclinical mastitis (SCM) and clinical mastitis (CLM) were identified by 16S rDNA and the sensitivity to both antibiotics and bacteriocins of Bacillus thuringiensis was tested.", "source": "pubmed", "title": "Molecular detection and sensitivity to antibiotics and bacteriocins of pathogens isolated from bovine mastitis in family dairy herds of central Mexico.", "url": "https://pubmed.ncbi.nlm.nih.gov/25815326/", "year": "2015", "id": "PMID:25815326" }, { "sentence": "Fecal NAS were isolated and then identified at the species level using transfer RNA-intergenic spacer PCR and sequencing of the 16S rRNA housekeeping gene.", "source": "pubmed", "title": "Non-aureus staphylococci in fecal samples of dairy cows: First report and phenotypic and genotypic characterization.", "url": "https://pubmed.ncbi.nlm.nih.gov/31421888/", "year": "2019", "id": "PMID:31421888" }, { "sentence": "Bacterial isolates recovered from cardiac lesions were identified as V. fluvialis using MALDI-TOF mass spectrometry and confirmed by 16S rRNA gene sequencing.", "source": "europepmc", "title": "Avian Infective Endocarditis Associated with <i>Vagococcus fluvialis</i>: A Case Report and Literature Review.", "url": "https://doi.org/10.3390/ani16081267", "year": "2026", "id": "MED:42072033 doi:10.3390/ani16081267" }, { "sentence": "Test Agreement among Biochemical Methods, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, and 16S rRNA Sequencing for Identification of Microorganisms Isolated from Bovine Milk..", "source": "europepmc", "title": "Test Agreement among Biochemical Methods, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, and 16S rRNA Sequencing for Identification of Microorganisms Isolated from Bovine Milk.", "url": "https://doi.org/10.1128/jcm.01381-18", "year": "2019", "id": "MED:30567749 doi:10.1128/jcm.01381-18" }, { "sentence": "Basic biochemical testing, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and 16S rRNA partial genome sequence analysis were compared for bacterial identification to the genus or species level.", "source": "europepmc", "title": "Test Agreement among Biochemical Methods, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, and 16S rRNA Sequencing for Identification of Microorganisms Isolated from Bovine Milk.", "url": "https://doi.org/10.1128/jcm.01381-18", "year": "2019", "id": "MED:30567749 doi:10.1128/jcm.01381-18" }, { "sentence": "Milk samples submitted from a commercial dairy farm from recently calved cows or clinical mastitis cases were cultured, and 181 isolates were identified by biochemical testing, MALDI-TOF MS, and 16S rRNA sequence analysis (179 isolates; 2 isolates could not be recovered from storage).", "source": "europepmc", "title": "Test Agreement among Biochemical Methods, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, and 16S rRNA Sequencing for Identification of Microorganisms Isolated from Bovine Milk.", "url": "https://doi.org/10.1128/jcm.01381-18", "year": "2019", "id": "MED:30567749 doi:10.1128/jcm.01381-18" } ], "inferred": false }, { "key": "dnase", "value": "DNase", "score": 3, "sources": [ "europepmc", "openalex" ], "n_sources": 2, "confidence": "low", "evidence": [ { "sentence": "Staphylococcus chromogenes, S. lentus, and S. epidermidis exhibited the highest DNase and/or gelatinase activity.", "source": "europepmc", "title": "Virulence factors and biofilm forming ability of Staphyloccoccus species isolated from skeletal lesions of broiler chickens.", "url": "https://doi.org/10.1038/s41598-025-95006-w", "year": "2025", "id": "MED:40155729 doi:10.1038/s41598-025-95006-w" }, { "sentence": "By polymerase chain reaction (PCR) analysis, the isolate, no. 605 that exhibited remarkable histamine‐degrading activity lacked the genes encoding coagulase and DNase, nor did it harbor any of the five classical enterotoxin genes.", "source": "openalex", "title": "Inhibition of histamine accumulation by novel histamine‐degrading species of Staphylococcus sp. isolated from goats and sheep milk", "url": "https://doi.org/10.1002/fsn3.2723", "year": "2022", "id": "https://openalex.org/W4206185474" } ], "inferred": false }, { "key": "whole_genome_sequencing_ani", "value": "Whole-genome sequencing / ANI", "score": 3, "sources": [ "europepmc", "openalex" ], "n_sources": 2, "confidence": "low", "evidence": [ { "sentence": "Methods: This study performed whole-genome sequencing and comparative genomic analysis of an ST398-MRSA-Vc isolate (NPREF115) from an RTE pork product in China.", "source": "europepmc", "title": "Evolutionary and Mobile Genetic Element Analysis of a Multidrug-Resistant ST398-MRSA-Vc Isolate from Ready-to-Eat Pork Products.", "url": "https://doi.org/10.3390/antibiotics15030314", "year": "2026", "id": "MED:41892475 doi:10.3390/antibiotics15030314" }, { "sentence": "The 16S rRNA sequence, MALDI-TOF mass spectrometry and positive urea hydrolysis and beta-glucuronidase tests clearly distinguished it from closely related Staphylococcus spp. All analyses have consistently shown that the closest relative is Staphylococcus chromogenes; however, values of digital DNA-DNA hybridization <35.3% and an average nucleotide identity <81.4% confirmed that the analyzed strain is a distinct Staphylococcus species.", "source": "openalex", "title": "Staphylococcus ratti sp. nov. Isolated from a Lab Rat", "url": "https://doi.org/10.3390/pathogens11010051", "year": "2022", "id": "https://openalex.org/W4206549958" } ], "inferred": false }, { "key": "gelatin_hydrolysis", "value": "Gelatin hydrolysis", "score": 2.07, "sources": [ "europepmc" ], "n_sources": 1, "confidence": "low", "evidence": [ { "sentence": "Staphylococcus chromogenes, S. lentus, and S. epidermidis exhibited the highest DNase and/or gelatinase activity.", "source": "europepmc", "title": "Virulence factors and biofilm forming ability of Staphyloccoccus species isolated from skeletal lesions of broiler chickens.", "url": "https://doi.org/10.1038/s41598-025-95006-w", "year": "2025", "id": "MED:40155729 doi:10.1038/s41598-025-95006-w" } ], "inferred": false }, { "key": "urease", "value": "Urease — reported positive", "score": 2.07, "sources": [ "openalex" ], "n_sources": 1, "confidence": "low", "evidence": [ { "sentence": "The 16S rRNA sequence, MALDI-TOF mass spectrometry and positive urea hydrolysis and beta-glucuronidase tests clearly distinguished it from closely related Staphylococcus spp. All analyses have consistently shown that the closest relative is Staphylococcus chromogenes; however, values of digital DNA-DNA hybridization <35.3% and an average nucleotide identity <81.4% confirmed that the analyzed strain is a distinct Staphylococcus species.", "source": "openalex", "title": "Staphylococcus ratti sp. nov. Isolated from a Lab Rat", "url": "https://doi.org/10.3390/pathogens11010051", "year": "2022", "id": "https://openalex.org/W4206549958" } ], "inferred": false }, { "key": "beta_glucuronidase_mug_", "value": "Beta-glucuronidase (MUG)", "score": 2.07, "sources": [ "openalex" ], "n_sources": 1, "confidence": "low", "evidence": [ { "sentence": "The 16S rRNA sequence, MALDI-TOF mass spectrometry and positive urea hydrolysis and beta-glucuronidase tests clearly distinguished it from closely related Staphylococcus spp. All analyses have consistently shown that the closest relative is Staphylococcus chromogenes; however, values of digital DNA-DNA hybridization <35.3% and an average nucleotide identity <81.4% confirmed that the analyzed strain is a distinct Staphylococcus species.", "source": "openalex", "title": "Staphylococcus ratti sp. nov. Isolated from a Lab Rat", "url": "https://doi.org/10.3390/pathogens11010051", "year": "2022", "id": "https://openalex.org/W4206549958" } ], "inferred": false }, { "key": "aerotolerance_test", "value": "Aerotolerance test — reported positive", "score": 2.07, "sources": [ "openalex" ], "n_sources": 1, "confidence": "low", "evidence": [ { "sentence": "Staphylococcus aureus is a gram-positive, facultative anaerobe coccus, which is a member of the commensal microbiota of skin and mucous membranes in humans and animals and sometimes can cause infection or disease.", "source": "openalex", "title": "Lameness caused by Staphylococcus aureus in poultry: A review", "url": "https://doi.org/10.61838/kman.jpsad.2.1.3", "year": "2024", "id": "https://openalex.org/W4393286254" } ], "inferred": false }, { "key": "vitek_2", "value": "VITEK 2", "score": 1.48, "sources": [ "europepmc" ], "n_sources": 1, "confidence": "low", "evidence": [ { "sentence": "From 180 samples, NAS were isolated using selective media and identified via the VITEK-2 system.", "source": "europepmc", "title": "Antimicrobial resistance profiles of non-aureus Staphylococci isolated from farm animals, farm environments and companion animals.", "url": "https://doi.org/10.1038/s41598-026-36455-9", "year": "2026", "id": "MED:41540231 doi:10.1038/s41598-026-36455-9" } ], "inferred": false }, { "key": "antibiotic_susceptibility_profile", "value": "Antibiotic susceptibility profile", "score": 0.93, "sources": [ "europepmc" ], "n_sources": 1, "confidence": "low", "evidence": [ { "sentence": "Antimicrobial susceptibility testing revealed a multidrug resistance profile, with resistance to several antimicrobial classes commonly used in poultry production.", "source": "europepmc", "title": "Avian Infective Endocarditis Associated with <i>Vagococcus fluvialis</i>: A Case Report and Literature Review.", "url": "https://doi.org/10.3390/ani16081267", "year": "2026", "id": "MED:42072033 doi:10.3390/ani16081267" } ], "inferred": false } ], "confidence": "high", "conflict": null, "review": "unreviewed" } }, "references": [ { "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9430782/", "year": "2022", "id": "PMC9430782", "source": "pmc", "fields": [ "media", "colony", "gram", "biochem", "time" ], "n": 19 }, { "title": "Species Identification and Strain Typing of Staphylococcus agnetis and Staphylococcus hyicus Isolates from Bovine Milk by Use of a Novel Multiplex PCR Assay and Pulsed-Field Gel Electrophoresis.", "url": "https://pubmed.ncbi.nlm.nih.gov/28330895/", "year": "2017", "id": "PMID:28330895", "source": "pubmed", "fields": [ "biochem" ], "n": 10 }, { "title": "Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk.", "url": "https://pubmed.ncbi.nlm.nih.gov/30799112/", "year": "2019", "id": "PMID:30799112", "source": "pubmed", "fields": [ "gram", "biochem" ], "n": 10 }, { "title": "Test Agreement among Biochemical Methods, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, and 16S rRNA Sequencing for Identification of Microorganisms Isolated from Bovine Milk.", "url": "https://doi.org/10.1128/jcm.01381-18", "year": "2019", "id": "MED:30567749 doi:10.1128/jcm.01381-18", "source": "europepmc", "fields": [ "biochem", "media" ], "n": 9 }, { "title": "Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR.", "url": "https://pubmed.ncbi.nlm.nih.gov/28532810/", "year": "2017", "id": "PMID:28532810", "source": "pubmed_pheno", "fields": [ "biochem" ], "n": 8 }, { "title": "Frequency of non-aureus staphylococci and mammaliicocci species isolated from quarter clinical mastitis: A 6-year retrospective study.", "url": "https://pubmed.ncbi.nlm.nih.gov/38056569/", "year": "2024", "id": "PMID:38056569", "source": "pubmed", "fields": [ "biochem" ], "n": 6 }, { "title": "Virulence factors and biofilm forming ability of Staphyloccoccus species isolated from skeletal lesions of broiler chickens.", "url": "https://doi.org/10.1038/s41598-025-95006-w", "year": "2025", "id": "MED:40155729 doi:10.1038/s41598-025-95006-w", "source": "europepmc", "fields": [ "biochem" ], "n": 6 }, { "title": "Species identification by MALDI-TOF MS and gap PCR–RFLP of non-aureus Staphylococcus, Mammaliicoccus, and Streptococcus spp. associated with sheep and goat mastitis", "url": "https://doi.org/10.1186/s13567-022-01102-4", "year": "2022", "id": "https://openalex.org/W4306291761", "source": "openalex", "fields": [ "biochem" ], "n": 6 }, { "title": "Staphylococcus ratti sp. nov. Isolated from a Lab Rat", "url": "https://doi.org/10.3390/pathogens11010051", "year": "2022", "id": "https://openalex.org/W4206549958", "source": "openalex", "fields": [ "biochem" ], "n": 6 }, { "title": "Draft genome sequence of Staphylococcus agnetis 4244, a strain with gene clusters encoding distinct post-translationally modified antimicrobial peptides.", "url": "https://pubmed.ncbi.nlm.nih.gov/34700053/", "year": "2021", "id": "PMID:34700053", "source": "pubmed", "fields": [ "gram", "media" ], "n": 4 }, { "title": "Avian Infective Endocarditis Associated with <i>Vagococcus fluvialis</i>: A Case Report and Literature Review.", "url": "https://doi.org/10.3390/ani16081267", "year": "2026", "id": "MED:42072033 doi:10.3390/ani16081267", "source": "europepmc", "fields": [ "biochem", "gram" ], "n": 4 }, { "title": "Draft genome sequence of Staphylococcus agnetis 4244, a strain with gene clusters encoding distinct post-translationally modified antimicrobial peptides", "url": "https://doi.org/10.1016/j.jgar.2021.10.006", "year": "2021", "id": "https://openalex.org/W3207983804", "source": "openalex", "fields": [ "gram", "media" ], "n": 4 }, { "title": "Draft genome sequence of Staphylococcus agnetis 4244, a strain with gene clusters encoding distinct post-translationally modified antimicrobial peptides", "url": "http://www.sciencedirect.com/science/article/pii/S2213716521002216", "year": "2021", "id": "881a8f1f0b614579b8a4686e9f37190c", "source": "doaj", "fields": [ "gram", "media" ], "n": 4 }, { "title": "Cross-sectional study to identify staphylococcal species isolated from teat and inguinal skin of different-aged dairy heifers.", "url": "https://pubmed.ncbi.nlm.nih.gov/29397170/", "year": "2018", "id": "PMID:29397170", "source": "pubmed", "fields": [ "media", "time", "biochem" ], "n": 3 }, { "title": "Diagnosing Intramammary Infection: Meta-Analysis and Mapping Review on Frequency and Udder Health Relevance of Microorganism Species Isolated from Bovine Milk Samples.", "url": "https://doi.org/10.3390/ani12233288", "year": "2022", "id": "MED:36496808 doi:10.3390/ani12233288", "source": "europepmc", "fields": [ "biochem" ], "n": 3 }, { "title": "Effects of cosmetic ingredients on growth and virulence factor expression in Staphylococcus aureus: a comparison between culture medium and in vitro skin model medium", "url": "https://doi.org/10.3934/microbiol.2025002", "year": "2024", "id": "doi:10.3934/microbiol.2025002", "source": "crossref", "fields": [ "media" ], "n": 3 }, { "title": "Short communication: Prevalence of methicillin resistance in coagulase-negative staphylococci and Staphylococcus aureus isolated from bulk milk on organic and conventional dairy farms in the United States", "url": "https://doi.org/10.3168/jds.2013-7523", "year": "2014", "id": "https://openalex.org/W2079380795", "source": "openalex", "fields": [ "biochem" ], "n": 3 }, { "title": "Inhibition of histamine accumulation by novel histamine‐degrading species of Staphylococcus sp. isolated from goats and sheep milk", "url": "https://doi.org/10.1002/fsn3.2723", "year": "2022", "id": "https://openalex.org/W4206185474", "source": "openalex", "fields": [ "time", "biochem" ], "n": 3 }, { "title": "Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle.", "url": "https://pubmed.ncbi.nlm.nih.gov/35863026/", "year": "2022", "id": "PMID:35863026", "source": "pubmed", "fields": [ "biochem" ], "n": 2 }, { "title": "Staphylococcus agnetis sp. nov., a coagulase-variable species from bovine subclinical and mild clinical mastitis.", "url": "https://pubmed.ncbi.nlm.nih.gov/21335502/", "year": "2012", "id": "PMID:21335502", "source": "pubmed", "fields": [ "gram", "biochem" ], "n": 2 }, { "title": "Molecular detection and sensitivity to antibiotics and bacteriocins of pathogens isolated from bovine mastitis in family dairy herds of central Mexico.", "url": "https://pubmed.ncbi.nlm.nih.gov/25815326/", "year": "2015", "id": "PMID:25815326", "source": "pubmed", "fields": [ "biochem" ], "n": 2 }, { "title": "Molecular characterization of non-aureus Staphylococcus spp. from heifer intramammary infections and body sites.", "url": "https://pubmed.ncbi.nlm.nih.gov/29525303/", "year": "2018", "id": "PMID:29525303", "source": "pubmed", "fields": [ "biochem" ], "n": 2 }, { "title": "Embryo lethality assay as a tool for assessing virulence of isolates from bacterial chondronecrosis with osteomyelitis in broilers", "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496174/", "year": "2021", "id": "PMC8496174", "source": "pmc", "fields": [ "media" ], "n": 2 }, { "title": "Prevalence of methicillin-resistant coagulase-negative staphylococci in Africa: a systematic review and meta-analysis.", "url": "https://doi.org/10.1186/s12879-025-11149-1", "year": "2025", "id": "MED:40640717 doi:10.1186/s12879-025-11149-1", "source": "europepmc", "fields": [ "biochem" ], "n": 2 }, { "title": "Species Profiles and Antimicrobial Resistance of Non-aureus Staphylococci Isolated from Healthy Broilers, Farm Environments, and Farm Workers.", "url": "https://doi.org/10.5851/kosfa.2023.e36", "year": "2023", "id": "MED:37701746 doi:10.5851/kosfa.2023.e36", "source": "europepmc", "fields": [ "biochem" ], "n": 2 }, { "title": "The role of Staphylococcus agnetis and Staphylococcus hyicus in the pathogenesis of buffalo fly skin lesions in cattle", "url": "https://doi.org/10.1101/2022.03.11.483979", "year": "2022", "id": "doi:10.1101/2022.03.11.483979", "source": "crossref", "fields": [ "biochem" ], "n": 2 }, { "title": "Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico", "url": "https://doi.org/10.1155/2015/615153", "year": "2015", "id": "https://openalex.org/W2056347332", "source": "openalex", "fields": [ "biochem" ], "n": 2 }, { "title": "Development and Validation of a Reference Data Set for Assigning Staphylococcus Species Based on Next-Generation Sequencing of the 16S-23S rRNA Region", "url": "https://doi.org/10.3389/fcimb.2019.00278", "year": "2019", "id": "https://openalex.org/W2963274849", "source": "openalex", "fields": [ "biochem" ], "n": 2 }, { "title": "De novo transcriptome analysis of halotolerant bacterium Staphylococcus sp. strain P-TSB-70 isolated from East coast of India: In search of salt stress tolerant genes", "url": "https://doi.org/10.1371/journal.pone.0228199", "year": "2020", "id": "https://openalex.org/W3005545504", "source": "openalex", "fields": [ "media" ], "n": 2 }, { "title": "Lameness caused by Staphylococcus aureus in poultry: A review", "url": "https://doi.org/10.61838/kman.jpsad.2.1.3", "year": "2024", "id": "https://openalex.org/W4393286254", "source": "openalex", "fields": [ "gram", "biochem" ], "n": 2 }, { "title": "Species Distribution, Antimicrobial Resistance, and Enterotoxin Profiles of Non-aureus Staphylococci Isolated from Poultry Slaughterhouses in Korea", "url": "https://doi.org/10.5851/kosfa.2024.e58", "year": "2025", "id": "573bbbd03741452fbb07812dd32b6450", "source": "doaj", "fields": [ "biochem" ], "n": 2 }, { "title": "Draft genome sequence of Staphylococcus agnetis 3682, the producing strain of the broad-spectrum lantibiotic agneticin 3682.", "url": "https://pubmed.ncbi.nlm.nih.gov/31454564/", "year": "2019", "id": "PMID:31454564", "source": "pubmed", "fields": [ "media" ], "n": 1 }, { "title": "Bovine-associated CNS species resist phagocytosis differently.", "url": "https://pubmed.ncbi.nlm.nih.gov/24207012/", "year": "2013", "id": "PMID:24207012", "source": "pubmed", "fields": [ "biochem" ], "n": 1 }, { "title": "Non-aureus staphylococci in fecal samples of dairy cows: First report and phenotypic and genotypic characterization.", "url": "https://pubmed.ncbi.nlm.nih.gov/31421888/", "year": "2019", "id": "PMID:31421888", "source": "pubmed", "fields": [ "biochem" ], "n": 1 }, { "title": "Species Profiles and Antimicrobial Resistance of Non- aureus Staphylococci Isolated from Healthy Broilers, Farm Environments, and Farm Workers.", "url": "https://pubmed.ncbi.nlm.nih.gov/37701746/", "year": "2023", "id": "PMID:37701746", "source": "pubmed", "fields": [ "biochem" ], "n": 1 }, { "title": "Distribution of non-aureus staphylococci species in udder quarters with low and high somatic cell count, and clinical mastitis.", "url": "https://pubmed.ncbi.nlm.nih.gov/28456402/", "year": "2017", "id": "PMID:28456402", "source": "pubmed", "fields": [ "biochem" ], "n": 1 }, { "title": "Antimicrobial resistance profiles of non-aureus Staphylococci isolated from farm animals, farm environments and companion animals.", "url": "https://doi.org/10.1038/s41598-026-36455-9", "year": "2026", "id": "MED:41540231 doi:10.1038/s41598-026-36455-9", "source": "europepmc", "fields": [ "biochem" ], "n": 1 }, { "title": "Assessment of Microbial and Heavy Metal Contamination of Natural Sheep Casings from Different 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