Culture profile
Clostridium fimetarium
cellular organisms; Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae; Lachnoclostridium
Built 25 Aug 2026 · 47 days ago
At a glance
Thioglycollate broth · Blood agar
35–37 °C
2 h
The PelA recombinant colonies were cultured and induced for expression for approximately 96 h in Pichia pastoris , as described by Mattanovich et al.
Gram-negative (rod, curved rod)
16S rRNA gene sequencing · Catalase — reported negative · Hydrogen sulfide (H2S) — reported positive +3 more
Evidence base by publication year
How current the underlying literature is. Recent work is weighted more heavily, but classical descriptions still count — that is often where the phenotype was characterised properly.
- Median 2016
- Newest 2025
- Oldest 1971
- Last 5 years 4
Latest literature
The newest publications that fed this profile.
- 2025
- 2024
- 2023
- 2022
- 2019
- 2016
1 Media used
low-
Thioglycollate broth
Evidence (3)
Generation time for strain 8797 was 1.5 times greater than previously reported for growth in purged fluid thioglycolate medium.
Chemically Defined Medium for the Growth of Clostridium perfringens — Crossref, 1971This medium consisted of a modification of the basal medium of Boyd et al. plus 0.1% sodium thioglycolate and 0.5% monosodium glutamate.
Chemically Defined Medium for Growth and Sporulation of Clostridium perfringens — Crossref, 1972The effects of glucose, monosodium glutamate, ammonium glutamate, and sodium thioglycolate on growth and sporulation of C. perfringens ATCC 12916 in the defined medium was investigated.
Chemically Defined Medium for Growth and Sporulation of Clostridium perfringens — Crossref, 1972
-
Blood agar
Evidence (1)
For this reason and due to the growing number of disorders associated with microbiota unbalance (dysbiosis or dysbacteriosis), the interest of several research groups has converged to the GI microbiota and its associations with human health.
Roles of Commensal Microbiota in Pancreas Homeostasis and Pancreatic Pathologies — PubMed Central (open access), 2015
2 Optimum temperature for growth
moderate35–37 °C
Evidence (6)
The optimum temperature for growth was 35-37 °C and the optimum pH was 6.7.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — PubMed (NCBI E-utilities), 2016The optimum temperature for growth was 35-37 °C and the optimum pH was 6.7.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — PubMed (NCBI E-utilities), 2016The optimum temperature for growth was 35-37 °C and the optimum pH was 6.7.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — Europe PMC, 2016The optimum temperature for growth was 35-37 °C and the optimum pH was 6.7.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — PubMed (NCBI E-utilities), 2016The optimum temperature for growth was 35-37 °C and the optimum pH was 6.7.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — PubMed (NCBI E-utilities), 2016The optimum temperature for growth was 35-37 °C and the optimum pH was 6.7.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — Europe PMC, 2016
Other values reported (4)
-
40 °C
Evidence (1)
To determine the optimal reaction temperature for PelA , a series of temperatures (40 °C, 45 °C, 50 °C, 55 °C, 60 °C, 65 °C, 70 °C, and 80 °C) were set, about 100 μg of protein per reaction was aliquoted, and the activity of PelA at these temperatures was measured.
Molecular Characterization of a Novel Alkaline Endo-Pectate Lyase from Paenibacillus borealis and Over-Production in Bioreactor Realized by Constructing the Tandem Expression Cassettes in Host Genome — PubMed Central (open access), 2025
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45 °C
Evidence (1)
To determine the optimal reaction temperature for PelA , a series of temperatures (40 °C, 45 °C, 50 °C, 55 °C, 60 °C, 65 °C, 70 °C, and 80 °C) were set, about 100 μg of protein per reaction was aliquoted, and the activity of PelA at these temperatures was measured.
Molecular Characterization of a Novel Alkaline Endo-Pectate Lyase from Paenibacillus borealis and Over-Production in Bioreactor Realized by Constructing the Tandem Expression Cassettes in Host Genome — PubMed Central (open access), 2025
-
50 °C
Evidence (1)
To determine the optimal reaction temperature for PelA , a series of temperatures (40 °C, 45 °C, 50 °C, 55 °C, 60 °C, 65 °C, 70 °C, and 80 °C) were set, about 100 μg of protein per reaction was aliquoted, and the activity of PelA at these temperatures was measured.
Molecular Characterization of a Novel Alkaline Endo-Pectate Lyase from Paenibacillus borealis and Over-Production in Bioreactor Realized by Constructing the Tandem Expression Cassettes in Host Genome — PubMed Central (open access), 2025
-
55 °C
Evidence (1)
To determine the optimal reaction temperature for PelA , a series of temperatures (40 °C, 45 °C, 50 °C, 55 °C, 60 °C, 65 °C, 70 °C, and 80 °C) were set, about 100 μg of protein per reaction was aliquoted, and the activity of PelA at these temperatures was measured.
Molecular Characterization of a Novel Alkaline Endo-Pectate Lyase from Paenibacillus borealis and Over-Production in Bioreactor Realized by Constructing the Tandem Expression Cassettes in Host Genome — PubMed Central (open access), 2025
3 Time required to grow
low2 h
Evidence (1)
Isolation and characterization of anaerobic microbes from marine environments in Korea. 유기산을 생산하는 발효미생물을 획득하기 위해 갯벌, 심해, 염전 등의 퇴적토와 해초시료 등의 시료에 대해 methanogen 배지, acetogen 배지, Clostridium용 배지 등을 이용하여 농후 배양을 실시하였다. 총 8개 시료로부터 65주의 혐기성 미생물을 분리하였으며 이 중 신규성이 높거나 활용성이 높다고 알려진 11종에 대해 계통분석, 성장 양식(growth pattern), 유기산 생산 평가 등을 시도하였다. 분석이 수행된 균주 중 Bacteroidia 강에 속하는 1주 외에는 모두 Clostridia 강에 속하였으며 성장속도는 $1.2h^{-1}$ 이상이었다. 분석이 수행된 7종 중 6종은 아세트산을 생성하였으며, 부가적으로 2균주는 부틸산을, 4균주는 개미산을 생산하였다.
Isolation and characterization of anaerobic microbes from marine environments in Korea — OpenAlex, 2016
Other values reported (4)
-
12 h
Evidence (1)
Correspondingly, to assess the pH stability of the enzyme, PelA was incubated in the aforementioned buffers for 4, 8, and 12 h, after which the remaining activity was measured.
Molecular Characterization of a Novel Alkaline Endo-Pectate Lyase from Paenibacillus borealis and Over-Production in Bioreactor Realized by Constructing the Tandem Expression Cassettes in Host Genome — PubMed Central (open access), 2025
-
96 h
Evidence (1)
The PelA recombinant colonies were cultured and induced for expression for approximately 96 h in Pichia pastoris , as described by Mattanovich et al. [ 30 ].
Molecular Characterization of a Novel Alkaline Endo-Pectate Lyase from Paenibacillus borealis and Over-Production in Bioreactor Realized by Constructing the Tandem Expression Cassettes in Host Genome — PubMed Central (open access), 2025
-
8 mo
Evidence (1)
Between FcGRIIb-/- and pristane, anti-dsDNA and TNF-α in FcGRIIb-/- were higher than pristane after 6 and 8 months old, respectively, while FcGRIIb-/- UPCI was higher than pristane at 10 months old, with a similar level of other parameters (Fig.
Comparative time-series analyses of gut microbiome profiles in genetically and chemically induced lupus-prone mice and the impacts of fecal transplantation — PubMed Central (open access), 2024
-
10 mo
Evidence (1)
Between FcGRIIb-/- and pristane, anti-dsDNA and TNF-α in FcGRIIb-/- were higher than pristane after 6 and 8 months old, respectively, while FcGRIIb-/- UPCI was higher than pristane at 10 months old, with a similar level of other parameters (Fig.
Comparative time-series analyses of gut microbiome profiles in genetically and chemically induced lupus-prone mice and the impacts of fecal transplantation — PubMed Central (open access), 2024
4 Colony morphology
lowThe PelA recombinant colonies were cultured and induced for expression for approximately 96 h in Pichia pastoris , as described by Mattanovich et al. [ 30 ].
Evidence (1)
The PelA recombinant colonies were cultured and induced for expression for approximately 96 h in Pichia pastoris , as described by Mattanovich et al. [ 30 ].
Molecular Characterization of a Novel Alkaline Endo-Pectate Lyase from Paenibacillus borealis and Over-Production in Bioreactor Realized by Constructing the Tandem Expression Cassettes in Host Genome — PubMed Central (open access), 2025
5 Gram character
moderateGram-negative (rod, curved rod)
Evidence (5)
Cells of the strain were Gram-stain-negative curved rods with a polar flagellum.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — PubMed (NCBI E-utilities), 2016Cells of the strain were Gram-stain-negative curved rods with a polar flagellum.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — PubMed (NCBI E-utilities), 2016The deranged function of the gut mucosal barrier and the presence of enteric Gram-negative bacteria in the pancreas suggest the participation of microbiota in the development of pancreatitis.
Roles of Commensal Microbiota in Pancreas Homeostasis and Pancreatic Pathologies — PubMed Central (open access), 2015Cells of the strain were Gram-stain-negative curved rods with a polar flagellum.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — Europe PMC, 2016This study isolated from river sediment two FRB, a Gram-negative strain (Geobaccter sp. LAR-2, GenBank no. KC211015) and a Gram-positive strain (Clostridium sp. LAR-3, GenBank no. KC211016), and individually started up MFC using these two isolates as inocula at anodic compartment.
Startup of Microbial Fuel Cells with Pure Ferric Iron-reducing Bacteria Isolates — OpenAlex, 2016
Other values reported (1)
-
Gram-positive (rod)
Evidence (3)
A new psychroactive saccharolytic anaerobic organism was isolated from cattle manure digested at 6 degrees C. The organism is a Gram-positive oligosporous motile rod measuring 0.5-0.6 by 2.1-5.0 microns.
[A new psychroactive bacterium, Clostridium fimentarium, isolated from cattle manure fermented at a low temperature]. — PubMed (NCBI E-utilities), 1995This study isolated from river sediment two FRB, a Gram-negative strain (Geobaccter sp. LAR-2, GenBank no. KC211015) and a Gram-positive strain (Clostridium sp. LAR-3, GenBank no. KC211016), and individually started up MFC using these two isolates as inocula at anodic compartment.
Startup of Microbial Fuel Cells with Pure Ferric Iron-reducing Bacteria Isolates — OpenAlex, 2016Inferred from NCBI Taxonomy lineage: cellular organisms; Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae; Lachnoclostridium
NCBI Taxonomy lineage — NCBI Taxonomy, 2026
6 Biochemical tests for identification
moderate-
16S rRNA gene sequencing
Evidence (5)
PCR amplification and sequencing of bacterial 16S rRNA genes (rDNA) with universally conserved primers have identified over 524 unique bacterial sequences affiliated with 12 bacterial divisions.
Partitioning of Bacterial Communities between Seawater and Healthy, Black Band Diseased, and Dead Coral Surfaces — PubMed Central (open access), 2002PCR amplification and sequencing of bacterial 16S rRNA genes (rDNA) with universally conserved primers have identified over 524 unique bacterial sequences affiliated with 12 bacterial divisions.
Partitioning of bacterial communities between seawater and healthy, black band diseased, and dead coral surfaces. — Europe PMC, 2002It appeared that combining probes generated by PCR amplification of the V1 and V6 variable regions of the 16S rRNA gene provided accurate differentiation of closely related organisms.
Microbial community analysis in sludge of anaerobic wastewater treatment systems : integrated culture-dependent and culture-independent approaches — OpenAlex, 2007From our enrichment performed under anaerobic condition, 65 strains were isolated and identified by the 16S rRNA gene sequence analysis.
Isolation and characterization of anaerobic microbes from marine environments in Korea — OpenAlex, 2016Here, we compared their composition and characteristics in 37 reproductive-aged Chinese women including 24 pregnant women with cervical incompetence (vaginal and cervical canal bacteria formed Groups A and B, respectively) and 13 healthy pregnant women (vaginal and cervical canal bacteria formed Groups C and D, respectively) using high-throughput sequencing of the V4 region of 16S rRNA gene.
Characterization of cervical canal and vaginal bacteria in pregnant women with cervical incompetence — DOAJ, 2022
-
Catalase — reported negative
Evidence (4)
Oxidase, catalase and nitrate-reducing activities were negative.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — PubMed (NCBI E-utilities), 2016Oxidase, catalase and nitrate-reducing activities were negative.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — PubMed (NCBI E-utilities), 2016Oxidase, catalase and nitrate-reducing activities were negative.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — Europe PMC, 2016It is catalase negative and grows only on sugars with the best growth rate being on lactose.
Anaerobic psychrophiles from Lake Zub and Lake Untersee, Antarctica — OpenAlex, 2009
-
Hydrogen sulfide (H2S) — reported positive
Evidence (3)
Hydrogen sulfide was produced.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — PubMed (NCBI E-utilities), 2016Hydrogen sulfide was produced.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — PubMed (NCBI E-utilities), 2016Hydrogen sulfide was produced.
Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. — Europe PMC, 2016
-
Short-chain fatty acid profile (GLC)
Evidence (1)
The increased abundance of butyrate-producing bacteria ( Clostridium fimetarium , Clostridium hylemona , and Sutterella wadsworthensis ) was correlated with an increase of the short-chain fatty acid, butyric acid after exposure to graphene.
Impact of Pristine Graphene on Intestinal Microbiota Assessed Using a Bioreactor-Rotary Cell Culture System. — Europe PMC, 2019
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Pigment production — reported positive
Evidence (1)
Exotic enzymes, cold-shock proteins and pigments produced by some of the extremophiles from polar environments have the potential to be of great benefit to Mankind.
Psychrophilic and Psychrotolerant Microbial Extremophiles in Polar Environments — OpenAlex, 2009
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Motility — reported positive
Evidence (1)
A new psychroactive saccharolytic anaerobic organism was isolated from cattle manure digested at 6 degrees C. The organism is a Gram-positive oligosporous motile rod measuring 0.5-0.6 by 2.1-5.0 microns.
[A new psychroactive bacterium, Clostridium fimentarium, isolated from cattle manure fermented at a low temperature]. — PubMed (NCBI E-utilities), 1995
7 References used
18 sourcesCatalogues to check by hand
These have no open interface, or their terms forbid automated querying. The app prepares the query; you open it.
Scan log — what each database returned
| Database | Status | Records | Evidence | Time | Note |
|---|---|---|---|---|---|
| NCBI Taxonomy — lineage and accepted name | empty | 0 | 0 | 1426 ms | TaxID 99656 (species). |
| LPSN — nomenclatural status | skipped | 0 | 0 | 0 ms | Skipped — LPSN credentials not configured (free registration). |
| StrainInfo — strain cross-references | empty | 0 | 0 | 1152 ms | No response (endpoint may require a different path). |
| GBIF — name resolution | empty | 0 | 0 | 672 ms | Matched as Clostridium fimetarium Kotsyurbenko et al., 1997 (ACCEPTED). |
| Wikidata — identifier bridge | empty | 0 | 0 | 897 ms | Item Q3681061 matched. |
| DSMZ MediaDive — curated growth media | empty | 0 | 0 | 691 ms | No media entries matched this name in MediaDive. |
| BacDive — curated strain phenotypes | skipped | 0 | 0 | 0 ms | Skipped — BacDive credentials not configured. This is the single highest-value source; registration is free. |
| PubMed — culture and cultivation query | hit | 2 | 7 | 1798 ms | 2 abstracts retrieved (culture query). |
| PubMed — morphology and identification query | hit | 1 | 5 | 2367 ms | 1 abstracts retrieved (pheno query). |
| PubMed Central — open-access Methods sections | hit | 5 | 18 | 7044 ms | 5 open-access full texts mined. |
| Europe PMC — abstract search | hit | 8 | 8 | 5960 ms | 8 records from Europe PMC. |
| Europe PMC — full-text mining | empty | 0 | 0 | 3323 ms | 0 full texts mined for Methods detail. |
| Crossref — DOI metadata | hit | 25 | 3 | 1142 ms | 25 DOI records (abstracts only where deposited). |
| OpenAlex — open scholarly graph | hit | 25 | 10 | 2471 ms | 25 works (inverted abstracts reconstructed locally). |
| Semantic Scholar — abstracts | empty | 0 | 0 | 1140 ms | Rate-limited or unavailable (keyless access is throttled). |
| DOAJ — open-access journals | hit | 1 | 1 | 720 ms | 1 open-access articles. |
| OpenAIRE — repository publications | empty | 0 | 0 | 818 ms | 0 repository publications. |
| SciELO — regional journals | empty | 0 | 0 | 566 ms | No parseable SciELO response (their JSON output is unstable; a deep link is still provided). |
| NCBI Bookshelf — reference texts | hit | 8 | 0 | 1334 ms | 8 reference-text chapters listed. |
| CORE — aggregated repositories | skipped | 0 | 0 | 0 ms | Skipped — no API key configured. |
| Springer Nature — publisher metadata | skipped | 0 | 0 | 0 ms | Skipped — no API key configured. |
| ScienceDirect — publisher metadata | skipped | 0 | 0 | 0 ms | Skipped — no API key configured. |
| bioRxiv / medRxiv — preprints | empty | 0 | 0 | 1550 ms | 0 preprints (not peer reviewed — weighted down). |
| Zenodo — datasets and protocols | empty | 0 | 0 | 706 ms | 0 deposits (datasets, theses, protocols). |
| Wikipedia — orientation only | empty | 0 | 0 | 966 ms | No article found. |
| ATCC — deep link | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |
| NCTC / UKHSA — deep link | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |
| Google Scholar — deep link (not crawled) | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |
| protocols.io — deep link | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |
| ResearchGate — deep link | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |