Culture profile
Staphylococcus agnetis
cellular organisms; Bacteria; Bacillati; Bacillota; Bacilli; Caryophanales; Staphylococcaceae; Staphylococcus
unreviewed
Built 12 Sep 2026 · 30 days ago
At a glance
1
Media
high
Brain heart infusion (BHI) · Tryptic soy broth (TSB) · Tryptic soy agar (TSA) +5 more
2
Temperature
low
37 °C
3
Growth time
high
24 h
4
Colony
low
Based on colony morphology, up to 4 representative colonies were selected from each plate, streaked onto tryptic soy agar plates (TSA, Sigma-Aldrich,
5
Gram
high
Gram-positive
6
Biochemical tests
high
Coagulase — reported positive · MALDI-TOF MS · Haemolysis pattern +9 more
Evidence base by publication year
How current the underlying literature is. Recent work is weighted more heavily, but classical descriptions still count — that is often where the phenotype was characterised properly.
- Median 2021
- Newest 2026
- Oldest 2012
- Last 5 years 30
Latest literature
The newest publications that fed this profile.
- 2026
- 2026
- 2026
- 2026
- 2025
- 2025
1 Media used
high-
Brain heart infusion (BHI)
Evidence (6)
The inhibitory activity of S. agnetis 3682 against 20 multidrug-resistant MRSA strains involved in human infections in two Brazilian hospitals was determined by the deferred antagonism assay on brain-heart infusion (BHI) agar plates.
Draft genome sequence of Staphylococcus agnetis 3682, the producing strain of the broad-spectrum lantibiotic agneticin 3682. — PubMed (NCBI E-utilities), 2019Investigation of the spectrum of activity of S. agnetis 4244 was performed on BHI agar by deferred antagonism assay.
Draft genome sequence of Staphylococcus agnetis 4244, a strain with gene clusters encoding distinct post-translationally modified antimicrobial peptides. — PubMed (NCBI E-utilities), 2021Here, we reconstituted a skin model using keratin as a base (instead of callus) and compared it with brain heart infusion (BHI) medium.
Effects of cosmetic ingredients on growth and virulence factor expression in <i>Staphylococcus aureus</i>: a comparison between culture medium and <i>in vitro</i> skin model medium — Crossref, 2024Upon the addition of cosmetic ingredients to BHI or skin model medium, the sensitivity of S. aureus to these cosmetic ingredients differed between the two media.
Effects of cosmetic ingredients on growth and virulence factor expression in <i>Staphylococcus aureus</i>: a comparison between culture medium and <i>in vitro</i> skin model medium — Crossref, 2024Notably, after adding the two tested cosmetic ingredients, the expression level of staphylococcal enterotoxin A in S. aureus reduced significantly in skin model medium compared with that in the BHI medium.
Effects of cosmetic ingredients on growth and virulence factor expression in <i>Staphylococcus aureus</i>: a comparison between culture medium and <i>in vitro</i> skin model medium — Crossref, 2024Investigation of the spectrum of activity of S. agnetis 4244 was performed on BHI agar by deferred antagonism assay.
Draft genome sequence of Staphylococcus agnetis 4244, a strain with gene clusters encoding distinct post-translationally modified antimicrobial peptides — OpenAlex, 2021
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Tryptic soy broth (TSB)
Evidence (4)
Mid log phase cultures (40 ml) in Tryptic Soy Broth (Difco, Becton, Dickinson and Company, Franklin Lakes, NJ) were pelleted and resuspended in 0.6 ml TE 10 mM TrisCl pH 7.5, 1 mM EDTA, 0.5% SDS.
Genome Analysis of Staphylococcus agnetis , an Agent of Lameness in Broiler Chickens — PubMed Central (open access), 2015A single well-isolated colony was selected from each TSA plate, grown in 9 mL of tryptic soy broth (TSB, Sigma-Aldrich, St.
Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens — PubMed Central (open access), 2023De novo transcriptome analysis of halotolerant bacterium Staphylococcus sp. strain P-TSB-70 isolated from East coast of India: In search of salt stress tolerant genes.
De novo transcriptome analysis of halotolerant bacterium Staphylococcus sp. strain P-TSB-70 isolated from East coast of India: In search of salt stress tolerant genes — OpenAlex, 2020In favour of whole-genome transcriptome profiling of Staphylococcus sp. strain P-TSB-70 (GenBank Accn. no. KP117091) which tolerated upto 20% NaCl stress, the strain was cultured in the laboratory condition with 20% NaCl stress.
De novo transcriptome analysis of halotolerant bacterium Staphylococcus sp. strain P-TSB-70 isolated from East coast of India: In search of salt stress tolerant genes — OpenAlex, 2020
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Tryptic soy agar (TSA)
Evidence (3)
The different culture media used were Todd-Hewitt, Tryptic Soy Agar, and culture medium containing peptone trypticase, 10 g/L; yeast extract, 1.0 g/L; KH 2 PO 4 , 3.0 g/L; K 2 HPO 4 , 4.8 g/L; (NH 4 ) 2 SO 4 , 30 g/L; MgSO 4 ·7H 2 O, 0.2 g/L; L-cysteine HCl·H 2 0, 0.5 g/L; sodium propionate, 15 g/L; agar, 15 g/L; pH 6.0-7.9, all with the addition of 5% of sheep blood.
Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico — PubMed Central (open access), 2015Based on colony morphology, up to 4 representative colonies were selected from each plate, streaked onto tryptic soy agar plates (TSA, Sigma-Aldrich, St.
Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens — PubMed Central (open access), 2023A single well-isolated colony was selected from each TSA plate, grown in 9 mL of tryptic soy broth (TSB, Sigma-Aldrich, St.
Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens — PubMed Central (open access), 2023
-
Nutrient broth
Evidence (1)
Competitive exclusion broth culture assay Staphylococcus and Bacillus DFM cultures were grown overnight as described above, co-inoculated at 10 5 and 10 6 CFU/mL respectively in Nutrient broth (ThermoFisher Scientific, Waltham, MA) and incubated with agitation (130 rpm) at 37 °C for 24 h ( 29 ).
Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens — PubMed Central (open access), 2023
-
Mannitol salt agar
Evidence (1)
Swabbing samples were mixed with 10 mL of sterile saline, agitated, and cultured on mannitol salt agar plates.
Cross-sectional study to identify staphylococcal species isolated from teat and inguinal skin of different-aged dairy heifers. — PubMed (NCBI E-utilities), 2018
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Blood agar
Evidence (1)
The objective of this prospective study was a blinded comparison of three methods for the identification of bacteria isolated on Columbia blood agar from milk samples of dairy cows.
Test Agreement among Biochemical Methods, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, and 16S rRNA Sequencing for Identification of Microorganisms Isolated from Bovine Milk. — Europe PMC, 2019
-
Buffered peptone water
Evidence (1)
After incubation, samples were serially diluted in 9 mL of buffered peptone water (BPW, Sigma-Aldrich, St.
Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens — PubMed Central (open access), 2023
-
Todd-Hewitt broth
Evidence (1)
The different culture media used were Todd-Hewitt, Tryptic Soy Agar, and culture medium containing peptone trypticase, 10 g/L; yeast extract, 1.0 g/L; KH 2 PO 4 , 3.0 g/L; K 2 HPO 4 , 4.8 g/L; (NH 4 ) 2 SO 4 , 30 g/L; MgSO 4 ·7H 2 O, 0.2 g/L; L-cysteine HCl·H 2 0, 0.5 g/L; sodium propionate, 15 g/L; agar, 15 g/L; pH 6.0-7.9, all with the addition of 5% of sheep blood.
Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico — PubMed Central (open access), 2015
2 Optimum temperature for growth
low37 °C
Evidence (3)
Louis, MO), spread plated onto CHROMagar Staphylococcus agar plates (CHROMagar, Paris, France), and incubated aerobically at 37 °C for 24 h.
Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens — PubMed Central (open access), 2023Competitive exclusion broth culture assay Staphylococcus and Bacillus DFM cultures were grown overnight as described above, co-inoculated at 10 5 and 10 6 CFU/mL respectively in Nutrient broth (ThermoFisher Scientific, Waltham, MA) and incubated with agitation (130 rpm) at 37 °C for 24 h ( 29 ).
Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens — PubMed Central (open access), 2023After incubation, co-cultures were serially diluted and plated onto CHROMagar Staphylococcus agar plates and incubated at 37 °C for 24 h for Staphylococcus enumeration.
Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens — PubMed Central (open access), 2023
3 Time required to grow
high24 h
Evidence (6)
At 24 h, plates were read and up to 10 staphylococcal colonies were saved for further analysis.
Cross-sectional study to identify staphylococcal species isolated from teat and inguinal skin of different-aged dairy heifers. — PubMed (NCBI E-utilities), 2018Fast growing and clinically healthy broilers showing no apparent gait abnormalities nevertheless can, within 24 hours, exhibit early symptoms of lameness (hesitancy to stand, eagerness to sit, slight wing-tip dipping).
Genome Analysis of Staphylococcus agnetis , an Agent of Lameness in Broiler Chickens — PubMed Central (open access), 2015Louis, MO), tubes were incubated aerobically at 37 °C for 24 h.
Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens — PubMed Central (open access), 2023Louis, MO), spread plated onto CHROMagar Staphylococcus agar plates (CHROMagar, Paris, France), and incubated aerobically at 37 °C for 24 h.
Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens — PubMed Central (open access), 2023Louis, MO), and incubated at 37 °C for 24 h.
Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens — PubMed Central (open access), 2023Louis, MO) and incubated at 37 °C for 24 h (overnight culture).
Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens — PubMed Central (open access), 2023
Other values reported (2)
-
72 h
Evidence (2)
The plates were incubated under aerobic conditions at 37 °C for 72 h.
Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico — PubMed Central (open access), 2015They also were subcultured in LB liquid at 37 °C for 72 h, and after this time 20% glycerol was added.
Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico — PubMed Central (open access), 2015
-
7 h
Evidence (1)
The different culture media used were Todd-Hewitt, Tryptic Soy Agar, and culture medium containing peptone trypticase, 10 g/L; yeast extract, 1.0 g/L; KH 2 PO 4 , 3.0 g/L; K 2 HPO 4 , 4.8 g/L; (NH 4 ) 2 SO 4 , 30 g/L; MgSO 4 ·7H 2 O, 0.2 g/L; L-cysteine HCl·H 2 0, 0.5 g/L; sodium propionate, 15 g/L; agar, 15 g/L; pH 6.0-7.9, all with the addition of 5% of sheep blood.
Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico — PubMed Central (open access), 2015
4 Colony morphology
lowBased on colony morphology, up to 4 representative colonies were selected from each plate, streaked onto tryptic soy agar plates (TSA, Sigma-Aldrich, St.
Evidence (1)
Based on colony morphology, up to 4 representative colonies were selected from each plate, streaked onto tryptic soy agar plates (TSA, Sigma-Aldrich, St.
Identification and characterization of the causative agents of Focal Ulcerative Dermatitis in commercial laying hens — PubMed Central (open access), 2023
Other values reported (2)
-
Colony morphologies were uniform and multiple colonies yielded the same species match.
Evidence (1)
Colony morphologies were uniform and multiple colonies yielded the same species match.
Genome Analysis of Staphylococcus agnetis , an Agent of Lameness in Broiler Chickens — PubMed Central (open access), 2015
-
Bacterial colonies were screened using a rapid boil method and PCR sequencing using Bact-8F and Bact-1391R primers to amplify and sequence nearly the entire 16S rDNA region.
Evidence (1)
Bacterial colonies were screened using a rapid boil method and PCR sequencing using Bact-8F and Bact-1391R primers to amplify and sequence nearly the entire 16S rDNA region.
Genome Analysis of Staphylococcus agnetis , an Agent of Lameness in Broiler Chickens — PubMed Central (open access), 2015
5 Gram character
highGram-positive
Evidence (7)
Thirteen Gram-positive-staining coagulase-variable staphylococci were isolated from subclinical and mild clinical mastitic bovine milk (n=12) and a teat apex (n=1).
Staphylococcus agnetis sp. nov., a coagulase-variable species from bovine subclinical and mild clinical mastitis. — PubMed (NCBI E-utilities), 2012Here we report the draft genome sequence of Staphylococcus agnetis 4244, a strain involved in bovine mastitis, and its ability to inhibit different species of antibiotic-resistant Gram-positive bacteria owing to bacteriocin production.
Draft genome sequence of Staphylococcus agnetis 4244, a strain with gene clusters encoding distinct post-translationally modified antimicrobial peptides. — PubMed (NCBI E-utilities), 2021Staphylococcus agnetis 4244 was able to inhibit all 44 strains of antibiotic-resistant Gram-positive bacteria tested in this study, including vancomycin-resistant enterococci (VRE), methicillin-resistant Staphylococcus aureus (MRSA) and other antibiotic-resistant staphylococcal strains.
Draft genome sequence of Staphylococcus agnetis 4244, a strain with gene clusters encoding distinct post-translationally modified antimicrobial peptides. — PubMed (NCBI E-utilities), 2021This study emphasises the potential biotechnological application of this strain for production of bacteriocins that could be used in the food industry as biopreservatives and/or in medicine as alternative therapeutic options against VRE, MRSA, vancomycin-intermediate S. aureus and other antibiotic-resistant Gram-positive bacteria, including biofilm-forming isolates.
Draft genome sequence of Staphylococcus agnetis 4244, a strain with gene clusters encoding distinct post-translationally modified antimicrobial peptides. — PubMed (NCBI E-utilities), 2021The term BLIS is applied to uncharacterized proteinaceous antimicrobials produced by gram-positive bacteria.
Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk. — PubMed (NCBI E-utilities), 2019All the gram-positive targets, including Bacillus cereus, Listeria monocytogenes, Enterococcus faecalis Ef37 (a tyramine-producer strain), Lactobacillus saerimneri 30a (a histamine-producer strain), and methicillin-resistant Staph. epidermidis, were inhibited by the Staph. chromogenes isolates.
Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk. — PubMed (NCBI E-utilities), 2019Inferred from NCBI Taxonomy lineage: cellular organisms; Bacteria; Bacillati; Bacillota; Bacilli; Caryophanales; Staphylococcaceae; Staphylococcus
NCBI Taxonomy lineage — NCBI Taxonomy, 2026
Other values reported (1)
-
Gram-negative
Evidence (3)
The antimicrobial compounds from test strains were not effective against gram-negative pathogens, including Salmonella enterica serovar Typhimurium, Escherichia coli O157:H7, and Klebsiella pneumonia as well as the indicator mold Aspergillus fumigatus.
Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk. — PubMed (NCBI E-utilities), 2019The antimicrobial compounds from test strains were not effective against gram-negative pathogens, including Salmonella enterica serovar Typhimurium, Escherichia coli O157:H7, and Klebsiella pneumonia as well as the indicator mold Aspergillus fumigatus.
Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk. — PubMed (NCBI E-utilities), 2019These regions contain orthologs to genes from various Gram-positive and Gram-negative bacteria [ 40 ].
A Review of Current Knowledge on Staphylococcus agnetis in Poultry — PubMed Central (open access), 2020
6 Biochemical tests for identification
high-
Coagulase — reported positive
Evidence (6)
Thirteen Gram-positive-staining coagulase-variable staphylococci were isolated from subclinical and mild clinical mastitic bovine milk (n=12) and a teat apex (n=1).
Staphylococcus agnetis sp. nov., a coagulase-variable species from bovine subclinical and mild clinical mastitis. — PubMed (NCBI E-utilities), 2012Staphylococcus hyicus and Staphylococcus agnetis are two coagulase-variable staphylococcal species that can be isolated from bovine milk and are difficult to differentiate.
Species Identification and Strain Typing of Staphylococcus agnetis and Staphylococcus hyicus Isolates from Bovine Milk by Use of a Novel Multiplex PCR Assay and Pulsed-Field Gel Electrophoresis. — PubMed (NCBI E-utilities), 2017The objectives of this study were to characterize isolates of bovine milk origin from a collection that had previously been characterized as coagulase-positive S. hyicus based on phenotypic species identification methods and to develop a PCR-based method for differentiating S. hyicus , S. agnetis , and Staphylococcus aureus Isolates ( n = 62) were selected from a previous study in which milk samples were collected from cows on 15 dairy herds.
Species Identification and Strain Typing of Staphylococcus agnetis and Staphylococcus hyicus Isolates from Bovine Milk by Use of a Novel Multiplex PCR Assay and Pulsed-Field Gel Electrophoresis. — PubMed (NCBI E-utilities), 2017Isolates were coagulase tested and identified to the species level using housekeeping gene sequencing.
Species Identification and Strain Typing of Staphylococcus agnetis and Staphylococcus hyicus Isolates from Bovine Milk by Use of a Novel Multiplex PCR Assay and Pulsed-Field Gel Electrophoresis. — PubMed (NCBI E-utilities), 2017Overall, 88% (37/42) of coagulase-positive S. agnetis isolates were found to be coagulase positive at 4 h.
Species Identification and Strain Typing of Staphylococcus agnetis and Staphylococcus hyicus Isolates from Bovine Milk by Use of a Novel Multiplex PCR Assay and Pulsed-Field Gel Electrophoresis. — PubMed (NCBI E-utilities), 2017The herd-level prevalence of coagulase-positive S. agnetis ranged from 0 to 2.17%.
Species Identification and Strain Typing of Staphylococcus agnetis and Staphylococcus hyicus Isolates from Bovine Milk by Use of a Novel Multiplex PCR Assay and Pulsed-Field Gel Electrophoresis. — PubMed (NCBI E-utilities), 2017
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MALDI-TOF MS
Evidence (6)
Bacterial identification was conducted by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) and strain typing by repetitive sequence-based PCR (rep-PCR) and DNA sequencing to determine species similarity and virulence factors.
Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle. — PubMed (NCBI E-utilities), 2022The data was comprised of microbiological results from quarter CM samples routinely submitted to Quality Milk Production Services at Cornell University for microbial identification by MALDI-TOF mass spectrometry.
Frequency of non-aureus staphylococci and mammaliicocci species isolated from quarter clinical mastitis: A 6-year retrospective study. — PubMed (NCBI E-utilities), 2024Bacterial identification was conducted by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) and strain typing by repetitive sequence-based PCR (rep-PCR) and DNA sequencing to determine species similarity and virulence factors.
Role of Staphylococcus agnetis and Staphylococcus hyicus in the Pathogenesis of Buffalo Fly Skin Lesions in Cattle. — PubMed (NCBI E-utilities), 2022The data was comprised of microbiological results from quarter CM samples routinely submitted to Quality Milk Production Services at Cornell University for microbial identification by MALDI-TOF mass spectrometry.
Frequency of non-aureus staphylococci and mammaliicocci species isolated from quarter clinical mastitis: A 6-year retrospective study. — PubMed (NCBI E-utilities), 2024Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR..
Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR. — PubMed (NCBI E-utilities), 2017Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples.
Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR. — PubMed (NCBI E-utilities), 2017
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Haemolysis pattern
Evidence (6)
The most common species identified from body site samples were S. chromogenes, S. xylosus, and Staphylococcus haemolyticus.
Molecular characterization of non-aureus Staphylococcus spp. from heifer intramammary infections and body sites. — PubMed (NCBI E-utilities), 2018Staphylococcus haemolyticus 4S12 was able to inhibit the majority of gram-positive bacteria.
Antimicrobial spectrum activity of bacteriocinogenic Staphylococcus strains isolated from goat and sheep milk. — PubMed (NCBI E-utilities), 2019Our results showed that 29 distinct NASM species were identified, with the 8 most prevalent NASM species being Staphylococcus chromogenes, Staphylococcus haemolyticus, Staphylococcus simulans, Staphylococcus epidermidis, Staphylococcus sciuri (now Mammaliicoccus sciuri), Staphylococcus agnetis/Staphylococcus hyicus, Staphylococcus borealis, and Staphylococcus xylosus.
Frequency of non-aureus staphylococci and mammaliicocci species isolated from quarter clinical mastitis: A 6-year retrospective study. — PubMed (NCBI E-utilities), 2024This study showed that Staphylococcus chromogenes remains the most frequent (43%) NASM species identified from bovine CM, followed by Staphylococcus haemolyticus (18%), and Staphylococcus simulans (12%).
Frequency of non-aureus staphylococci and mammaliicocci species isolated from quarter clinical mastitis: A 6-year retrospective study. — PubMed (NCBI E-utilities), 2024While two mecA -positive Staphylococcus epidermidis isolates from farm workers had SCC mec II and IV, a mecA -positive S. epidermidis isolate from broiler and a Staphylococcus haemolyticus isolate farm environment carried SCC mec V. The occurrence of multidrug resistance was observed in 48.1% (104/216 isolates) of NAS isolates with high resistance rates to β-lactams (>40%) and fusidic acid (59.7%).
Species Profiles and Antimicrobial Resistance of Non- aureus Staphylococci Isolated from Healthy Broilers, Farm Environments, and Farm Workers. — PubMed (NCBI E-utilities), 2023Overall, the most common species identified were Staphylococcus haemolyticus followed by Staphylococcus chromogenes, Staphylococcus xylosus, Staphylococcus devriesei, and Staphylococcus sciuri.
Cross-sectional study to identify staphylococcal species isolated from teat and inguinal skin of different-aged dairy heifers. — PubMed (NCBI E-utilities), 2018
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16S rRNA gene sequencing
Evidence (6)
Pathogens from bovine subclinical mastitis (SCM) and clinical mastitis (CLM) were identified by 16S rDNA and the sensitivity to both antibiotics and bacteriocins of Bacillus thuringiensis was tested.
Molecular detection and sensitivity to antibiotics and bacteriocins of pathogens isolated from bovine mastitis in family dairy herds of central Mexico. — PubMed (NCBI E-utilities), 2015Fecal NAS were isolated and then identified at the species level using transfer RNA-intergenic spacer PCR and sequencing of the 16S rRNA housekeeping gene.
Non-aureus staphylococci in fecal samples of dairy cows: First report and phenotypic and genotypic characterization. — PubMed (NCBI E-utilities), 2019Bacterial isolates recovered from cardiac lesions were identified as V. fluvialis using MALDI-TOF mass spectrometry and confirmed by 16S rRNA gene sequencing.
Avian Infective Endocarditis Associated with <i>Vagococcus fluvialis</i>: A Case Report and Literature Review. — Europe PMC, 2026Test Agreement among Biochemical Methods, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, and 16S rRNA Sequencing for Identification of Microorganisms Isolated from Bovine Milk..
Test Agreement among Biochemical Methods, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, and 16S rRNA Sequencing for Identification of Microorganisms Isolated from Bovine Milk. — Europe PMC, 2019Basic biochemical testing, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and 16S rRNA partial genome sequence analysis were compared for bacterial identification to the genus or species level.
Test Agreement among Biochemical Methods, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, and 16S rRNA Sequencing for Identification of Microorganisms Isolated from Bovine Milk. — Europe PMC, 2019Milk samples submitted from a commercial dairy farm from recently calved cows or clinical mastitis cases were cultured, and 181 isolates were identified by biochemical testing, MALDI-TOF MS, and 16S rRNA sequence analysis (179 isolates; 2 isolates could not be recovered from storage).
Test Agreement among Biochemical Methods, Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, and 16S rRNA Sequencing for Identification of Microorganisms Isolated from Bovine Milk. — Europe PMC, 2019
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DNase
Evidence (2)
Staphylococcus chromogenes, S. lentus, and S. epidermidis exhibited the highest DNase and/or gelatinase activity.
Virulence factors and biofilm forming ability of Staphyloccoccus species isolated from skeletal lesions of broiler chickens. — Europe PMC, 2025By polymerase chain reaction (PCR) analysis, the isolate, no. 605 that exhibited remarkable histamine‐degrading activity lacked the genes encoding coagulase and DNase, nor did it harbor any of the five classical enterotoxin genes.
Inhibition of histamine accumulation by novel histamine‐degrading species of Staphylococcus sp. isolated from goats and sheep milk — OpenAlex, 2022
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Whole-genome sequencing / ANI
Evidence (2)
Methods: This study performed whole-genome sequencing and comparative genomic analysis of an ST398-MRSA-Vc isolate (NPREF115) from an RTE pork product in China.
Evolutionary and Mobile Genetic Element Analysis of a Multidrug-Resistant ST398-MRSA-Vc Isolate from Ready-to-Eat Pork Products. — Europe PMC, 2026The 16S rRNA sequence, MALDI-TOF mass spectrometry and positive urea hydrolysis and beta-glucuronidase tests clearly distinguished it from closely related Staphylococcus spp. All analyses have consistently shown that the closest relative is Staphylococcus chromogenes; however, values of digital DNA-DNA hybridization <35.3% and an average nucleotide identity <81.4% confirmed that the analyzed strain is a distinct Staphylococcus species.
Staphylococcus ratti sp. nov. Isolated from a Lab Rat — OpenAlex, 2022
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Gelatin hydrolysis
Evidence (1)
Staphylococcus chromogenes, S. lentus, and S. epidermidis exhibited the highest DNase and/or gelatinase activity.
Virulence factors and biofilm forming ability of Staphyloccoccus species isolated from skeletal lesions of broiler chickens. — Europe PMC, 2025
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Urease — reported positive
Evidence (1)
The 16S rRNA sequence, MALDI-TOF mass spectrometry and positive urea hydrolysis and beta-glucuronidase tests clearly distinguished it from closely related Staphylococcus spp. All analyses have consistently shown that the closest relative is Staphylococcus chromogenes; however, values of digital DNA-DNA hybridization <35.3% and an average nucleotide identity <81.4% confirmed that the analyzed strain is a distinct Staphylococcus species.
Staphylococcus ratti sp. nov. Isolated from a Lab Rat — OpenAlex, 2022
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Beta-glucuronidase (MUG)
Evidence (1)
The 16S rRNA sequence, MALDI-TOF mass spectrometry and positive urea hydrolysis and beta-glucuronidase tests clearly distinguished it from closely related Staphylococcus spp. All analyses have consistently shown that the closest relative is Staphylococcus chromogenes; however, values of digital DNA-DNA hybridization <35.3% and an average nucleotide identity <81.4% confirmed that the analyzed strain is a distinct Staphylococcus species.
Staphylococcus ratti sp. nov. Isolated from a Lab Rat — OpenAlex, 2022
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Aerotolerance test — reported positive
Evidence (1)
Staphylococcus aureus is a gram-positive, facultative anaerobe coccus, which is a member of the commensal microbiota of skin and mucous membranes in humans and animals and sometimes can cause infection or disease.
Lameness caused by Staphylococcus aureus in poultry: A review — OpenAlex, 2024
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VITEK 2
Evidence (1)
From 180 samples, NAS were isolated using selective media and identified via the VITEK-2 system.
Antimicrobial resistance profiles of non-aureus Staphylococci isolated from farm animals, farm environments and companion animals. — Europe PMC, 2026
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Antibiotic susceptibility profile
Evidence (1)
Antimicrobial susceptibility testing revealed a multidrug resistance profile, with resistance to several antimicrobial classes commonly used in poultry production.
Avian Infective Endocarditis Associated with <i>Vagococcus fluvialis</i>: A Case Report and Literature Review. — Europe PMC, 2026
7 References used
54 sourcesCatalogues to check by hand
These have no open interface, or their terms forbid automated querying. The app prepares the query; you open it.
Scan log — what each database returned
| Database | Status | Records | Evidence | Time | Note |
|---|---|---|---|---|---|
| NCBI Taxonomy — lineage and accepted name | empty | 0 | 0 | 1353 ms | TaxID 985762 (species). |
| LPSN — nomenclatural status | skipped | 0 | 0 | 0 ms | Skipped — LPSN credentials not configured (free registration). |
| StrainInfo — strain cross-references | empty | 0 | 0 | 1041 ms | No response (endpoint may require a different path). |
| GBIF — name resolution | empty | 0 | 0 | 684 ms | Matched as Staphylococcus agnetis Taponen et al., 2012 (ACCEPTED). |
| Wikidata — identifier bridge | empty | 0 | 0 | 894 ms | Item Q20720510 matched. |
| DSMZ MediaDive — curated growth media | empty | 0 | 0 | 1116 ms | No media entries matched this name in MediaDive. |
| BacDive — curated strain phenotypes | skipped | 0 | 0 | 0 ms | Skipped — BacDive credentials not configured. This is the single highest-value source; registration is free. |
| PubMed — culture and cultivation query | hit | 25 | 36 | 2606 ms | 25 abstracts retrieved (culture query). |
| PubMed — morphology and identification query | hit | 9 | 23 | 2084 ms | 9 abstracts retrieved (pheno query). |
| PubMed Central — open-access Methods sections | hit | 5 | 28 | 7543 ms | 5 open-access full texts mined. |
| Europe PMC — abstract search | hit | 25 | 30 | 1704 ms | 25 records from Europe PMC. |
| Europe PMC — full-text mining | empty | 0 | 0 | 3123 ms | 0 full texts mined for Methods detail. |
| Crossref — DOI metadata | hit | 25 | 4 | 1572 ms | 25 DOI records (abstracts only where deposited). |
| OpenAlex — open scholarly graph | hit | 25 | 37 | 2611 ms | 25 works (inverted abstracts reconstructed locally). |
| Semantic Scholar — abstracts | empty | 0 | 0 | 1148 ms | Rate-limited or unavailable (keyless access is throttled). |
| DOAJ — open-access journals | hit | 13 | 8 | 697 ms | 13 open-access articles. |
| OpenAIRE — repository publications | empty | 0 | 0 | 921 ms | 0 repository publications. |
| SciELO — regional journals | empty | 0 | 0 | 486 ms | No parseable SciELO response (their JSON output is unstable; a deep link is still provided). |
| NCBI Bookshelf — reference texts | empty | 0 | 0 | 656 ms | No reference-text chapters matched. |
| CORE — aggregated repositories | skipped | 0 | 0 | 0 ms | Skipped — no API key configured. |
| Springer Nature — publisher metadata | skipped | 0 | 0 | 0 ms | Skipped — no API key configured. |
| ScienceDirect — publisher metadata | skipped | 0 | 0 | 0 ms | Skipped — no API key configured. |
| bioRxiv / medRxiv — preprints | hit | 4 | 1 | 1031 ms | 4 preprints (not peer reviewed — weighted down). |
| Zenodo — datasets and protocols | empty | 0 | 0 | 683 ms | 0 deposits (datasets, theses, protocols). |
| Wikipedia — orientation only | hit | 1 | 2 | 1611 ms | Article retrieved (lowest weight). |
| ATCC — deep link | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |
| NCTC / UKHSA — deep link | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |
| Google Scholar — deep link (not crawled) | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |
| protocols.io — deep link | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |
| ResearchGate — deep link | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |