Culture profile
Stenotrophomonas acidaminiphila
cellular organisms; Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Lysobacterales; Lysobacteraceae; Stenotrophomonas
unreviewed
Built 10 Oct 2026 · yesterday
At a glance
1
Media
moderate
Basal salts / minimal medium · Luria-Bertani (LB) · Tryptic soy broth (TSB) +2 more
2
Temperature
moderate
55–75 °C
3
Growth time
moderate
5 d
4
Colony
low
The colonies of strain BJ1 on LB plate were round with edge neat, uplifted surface, opaque and yellow in colour.
5
Gram
high
Gram-negative
6
Biochemical tests
high
16S rRNA gene sequencing · Whole-genome sequencing / ANI — reported positive · MALDI-TOF MS +11 more
Evidence base by publication year
How current the underlying literature is. Recent work is weighted more heavily, but classical descriptions still count — that is often where the phenotype was characterised properly.
- Median 2022
- Newest 2026
- Oldest 2002
- Last 5 years 34
Latest literature
The newest publications that fed this profile.
- 2026
- 2026
- 2026
- 2026
- 2026
- 2026
1 Media used
moderate-
Basal salts / minimal medium
Evidence (6)
The strain JS-1 exhibited substantial growth in M9 mineral salt medium supplemented with 3.2 mmol l(-1) butachlor, as a sole source of carbon and energy.
Isolation and characterization of butachlor-catabolizing bacterial strain Stenotrophomonas acidaminiphila JS-1 from soil and assessment of its biodegradation potential. — PubMed (NCBI E-utilities), 2010The genes for glycerol utilization were detected in the genomes of APG2 and APG4, which corroborated with their ability to grow on a minimal medium containing glycerol as the sole co-substrate.
Genome analysis to decipher syntrophy in the bacterial consortium 'SCP' for azo dye degradation. — PubMed (NCBI E-utilities), 2021The strain JS-1 exhibited substantial growth in M9 mineral salt medium supplemented with 3.2 mmol l(-1) butachlor, as a sole source of carbon and energy.
Isolation and characterization of butachlor-catabolizing bacterial strain Stenotrophomonas acidaminiphila JS-1 from soil and assessment of its biodegradation potential. — PubMed (NCBI E-utilities), 2010Results The genes for glycerol utilization were detected in the genomes of APG2 and APG4, which corroborated with their ability to grow on a minimal medium containing glycerol as the sole co-substrate.
Genome analysis to decipher syntrophy in the bacterial consortium ‘SCP’ for azo dye degradation — PubMed Central (open access), 2021In the submerged fermentation, minimal medium was dispensed as 20 mL into 100 mL Erlenmeyer flasks in triplicates.
Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila — PubMed Central (open access), 2022Aliquots of 5 g were used for pre-culture in 500 mL Erlenmeyer flasks containing 100 mL of minimal medium ( Dellamatrice and Monteiro, 2004 ) composed of (g L -1 ) 6.0 of NaNO 3 , 1.5 of KH 2 PO 4 , 0.5 of KCl, 0.5 of MgSO 4 ·7H 2 O, 0.001 of FeSO 4 , 0.001 of ZnSO 4 , at pH 6.8.
Diuron degradation by bacteria from soil of sugarcane crops — PubMed Central (open access), 2017
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Luria-Bertani (LB)
Evidence (6)
Maximum specific growth rate (µ max = 0.362 h -1 ) of the bacterium in the optimized medium did not differ significantly from those in Luria-Bertani and trypticase soy broths.
A novel strain of Stenotrophomonas acidaminiphila produces thermostable alkaline peptidase on agro-industrial wastes: process optimization, kinetic modeling and scale-up. — PubMed (NCBI E-utilities), 2022Materials and methods Experimental materials The main reagents used in the experiment, LB medium, NaCl, and H 2 SO 4 , were purchased from Sinopharm Chemical Reagent Co., Ltd.
Two types of microorganisms isolated from petroleum hydrocarbon pollutants: Degradation characteristics and metabolic pathways analysis of petroleum hydrocarbons — PubMed Central (open access), 2024For decolorization studies, the three isolates were grown in Luria Bertani Broth for overnight at 37 °C under the shaking condition (i.e., 150 rpm).
Genome analysis to decipher syntrophy in the bacterial consortium ‘SCP’ for azo dye degradation — PubMed Central (open access), 2021The three bacterial isolates were otherwise maintained as pure cultures on Luria Bertani agar.
Genome analysis to decipher syntrophy in the bacterial consortium ‘SCP’ for azo dye degradation — PubMed Central (open access), 2021Flasks were sterilized by autoclaving and supplemented with filter-sterilized skimmed milk after cooling and subsequently inoculated with 3% (vv -1 ) 18 h-old Luria-Bertani (LB) broth of all improved mutants.
Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila — PubMed Central (open access), 2022The bacterial growth medium (Luria-Bertani, LB) contained 10.0 g l -1 tryptone, 5.0 g l -1 yeast extract, 1.0 g l -1 NaCl (pH 7.0).
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — PubMed Central (open access), 2019
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Tryptic soy broth (TSB)
Evidence (3)
Maximum specific growth rate (µ max = 0.362 h -1 ) of the bacterium in the optimized medium did not differ significantly from those in Luria-Bertani and trypticase soy broths.
A novel strain of Stenotrophomonas acidaminiphila produces thermostable alkaline peptidase on agro-industrial wastes: process optimization, kinetic modeling and scale-up. — PubMed (NCBI E-utilities), 2022Heavy metal tolerance to lead (Pb), cadmium (Cd), and mercury (Hg) was evaluated in tryptic soy broth (TSB) containing up to 9,000 ppm of each metal.
Scaling EPS yield and bio-carrier application of <i>Bacillus paralicheniformis</i> UB08 for rapid lead remediation. — Europe PMC, 2026Maximum specific growth rate (µ max = 0.362 h -1 ) of the bacterium in the optimized medium did not differ significantly from those in Luria-Bertani and trypticase soy broths.
A novel strain of Stenotrophomonas acidaminiphila produces thermostable alkaline peptidase on agro-industrial wastes: process optimization, kinetic modeling and scale-up. — Europe PMC, 2022
-
Tryptic soy agar (TSA)
Evidence (1)
In both cases, irradiated cells were serially diluted (10-fold) in normal saline (0.90% NaCl) and plated on trypticase soy agar (TSA) by the spread plate technique and incubated at 30 °C for 48 h in the dark to obtain viable cells.
Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila — PubMed Central (open access), 2022
-
Nutrient broth
Evidence (1)
Six highly copper-tolerant isolates named as MYS1, MYS2, MYS3, MYS4, MYS5 and MYS6 were isolated through enrichment in copper containing nutrient broth.
Copper-tolerant rhizosphere bacteria-characterization and assessment of plant growth promoting factors. — PubMed (NCBI E-utilities), 2017
2 Optimum temperature for growth
moderate55–75 °C
Evidence (6)
4 a shows that optimum temperature for protease activity ranged from 55 to 75 °C making it thermo-stable; the stability of which lasted for 60 min at 55-65 °C.
Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila — PubMed Central (open access), 20224 a shows that optimum temperature for protease activity ranged from 55 to 75 °C making it thermo-stable; the stability of which lasted for 60 min at 55-65 °C.
Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila — PubMed Central (open access), 2022Many Bacillus proteases have activity optima lower than 75 °C giving the protease in this study significant advantage.
Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila — PubMed Central (open access), 2022Enzyme kinetic studies indicated optimal laccase activity at temperatures of 45-55 °C and pH levels of 4-7, with isolates TR32, SH2A, and AK17 producing laccase activities exceeding 90 U/L. Molecular characterization via 16 S rRNA sequencing identified these isolates as belonging to genera such as Pseudomonas, Enterobacter, Acinetobacter, and Klebsiella, known for their lignocellulolytic enzyme production.
Bio decomposition of paddy straw waste using laccase producing novel soil bacterial isolates: An eco-friendly and sustainable approach. — Europe PMC, 2026Optimization in modified TSB (MTSB), containing 0.6% sucrose and 4% yeast extract at pH 6.0 and 55 °C, enhanced EPS production 5.5-fold to 0.4524 ± 0.0176 g/100 ml compared with 0.0821 ± 0.0076 g/100 ml in standard TSB.
Scaling EPS yield and bio-carrier application of <i>Bacillus paralicheniformis</i> UB08 for rapid lead remediation. — Europe PMC, 20264 a shows that optimum temperature for protease activity ranged from 55 to 75 °C making it thermo-stable; the stability of which lasted for 60 min at 55-65 °C.
Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila — PubMed Central (open access), 2022
Other values reported (4)
-
37.2 °C
Evidence (4)
The optimum conditions for DBP degradation were determined by response surface methodology (RSM) analysis to be 30.9 mg/l DBP concentration, pH 7.5, at a culture temperature of 37.2 °C.
Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with Citrobacter freundii CD-9 to Degrade Fenvalerate. — PubMed (NCBI E-utilities), 2022Proteolytic activity was evaluated using azocasein and casein assays after cultivation in an optimized medium containing 1% soybean paste as an inducer at 37 °C and 120 rpm for 72 h.
Bioprospecting of Aerobic Bacteria with Proteolytic Potential Isolated from Animal and Water Sources in the Three Regions of Mainland Ecuador. — Europe PMC, 2026The strain efficiently degraded 300 ng/mL of OTA within 72 h (98% degradation) under optimal conditions of 37 °C, pH 5.0, and 180 rpm.
Detoxification of Ochratoxin a by <i>Weizmannia coagulans</i> CGMCC 9951: Characterization, Mechanism, and Application in <i>Cornus officinalis</i> Pulp. — Europe PMC, 2026The optimum conditions for DBP degradation were determined by response surface methodology (RSM) analysis to be 30.9 mg/l DBP concentration, pH 7.5, at a culture temperature of 37.2 °C.
Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with <i>Citrobacter freundii</i> CD-9 to Degrade Fenvalerate. — Europe PMC, 2022
-
55–65 °C
Evidence (4)
4 a shows that optimum temperature for protease activity ranged from 55 to 75 °C making it thermo-stable; the stability of which lasted for 60 min at 55-65 °C.
Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila — PubMed Central (open access), 2022Enzyme kinetic studies indicated optimal laccase activity at temperatures of 45-55 °C and pH levels of 4-7, with isolates TR32, SH2A, and AK17 producing laccase activities exceeding 90 U/L. Molecular characterization via 16 S rRNA sequencing identified these isolates as belonging to genera such as Pseudomonas, Enterobacter, Acinetobacter, and Klebsiella, known for their lignocellulolytic enzyme production.
Bio decomposition of paddy straw waste using laccase producing novel soil bacterial isolates: An eco-friendly and sustainable approach. — Europe PMC, 2026Optimization in modified TSB (MTSB), containing 0.6% sucrose and 4% yeast extract at pH 6.0 and 55 °C, enhanced EPS production 5.5-fold to 0.4524 ± 0.0176 g/100 ml compared with 0.0821 ± 0.0076 g/100 ml in standard TSB.
Scaling EPS yield and bio-carrier application of <i>Bacillus paralicheniformis</i> UB08 for rapid lead remediation. — Europe PMC, 20264 a shows that optimum temperature for protease activity ranged from 55 to 75 °C making it thermo-stable; the stability of which lasted for 60 min at 55-65 °C.
Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila — PubMed Central (open access), 2022
-
30 °C
Evidence (4)
At initial concentrations of 50, 100, 200 and 300 mg l -1 , it degraded 91.5%, 89.4%, 86.5% and 83.5% of chlorothalonil after 96 h of inoculation under optimum conditions (30 °C and pH 7.0).
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil. — PubMed (NCBI E-utilities), 2019At initial concentrations of 50, 100, 200 and 300 mg l -1 , it degraded 91.5%, 89.4%, 86.5% and 83.5% of chlorothalonil after 96 h of inoculation under optimum conditions (30 °C and pH 7.0).
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — Crossref, 2019At initial concentrations of 50, 100, 200 and 300 mg l-1, it degraded 91.5%, 89.4%, 86.5% and 83.5% of chlorothalonil after 96 h of inoculation under optimum conditions (30 °C and pH 7.0).
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — DOAJ, 2019At initial concentrations of 50, 100, 200, 300 mg l-1, it degraded 91.5%, 89.4%, 86.5 and 83.5% of chlorothalonil after 96 h of inoculation under optimum conditions (30 °C and pH 7.0), respectively.
Data from: Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — Zenodo, 2019
-
45–55 °C
Evidence (3)
Enzyme kinetic studies indicated optimal laccase activity at temperatures of 45-55 °C and pH levels of 4-7, with isolates TR32, SH2A, and AK17 producing laccase activities exceeding 90 U/L. Molecular characterization via 16 S rRNA sequencing identified these isolates as belonging to genera such as Pseudomonas, Enterobacter, Acinetobacter, and Klebsiella, known for their lignocellulolytic enzyme production.
Bio decomposition of paddy straw waste using laccase producing novel soil bacterial isolates: An eco-friendly and sustainable approach. — Europe PMC, 2026Enzyme kinetic studies indicated optimal laccase activity at temperatures of 45-55 °C and pH levels of 4-7, with isolates TR32, SH2A, and AK17 producing laccase activities exceeding 90 U/L. Molecular characterization via 16 S rRNA sequencing identified these isolates as belonging to genera such as Pseudomonas, Enterobacter, Acinetobacter, and Klebsiella, known for their lignocellulolytic enzyme production.
Bio decomposition of paddy straw waste using laccase producing novel soil bacterial isolates: An eco-friendly and sustainable approach. — Europe PMC, 2026Optimization in modified TSB (MTSB), containing 0.6% sucrose and 4% yeast extract at pH 6.0 and 55 °C, enhanced EPS production 5.5-fold to 0.4524 ± 0.0176 g/100 ml compared with 0.0821 ± 0.0076 g/100 ml in standard TSB.
Scaling EPS yield and bio-carrier application of <i>Bacillus paralicheniformis</i> UB08 for rapid lead remediation. — Europe PMC, 2026
Sources disagree: "55–75 °C" (score 7.14) versus "37.2 °C" (score 5.59). Both are shown; a reviewer should decide.
3 Time required to grow
moderate5 d
Evidence (6)
Y4B demonstrated strong competitiveness and substantially accelerated the degradation of glyphosate in different soils, degrading 71.93 and 89.81% of glyphosate (400 mg kg -1 ) within 5 days in sterile and nonsterile soils, respectively.
Effects of Free or Immobilized Bacterium Stenotrophomonas acidaminiphila Y4B on Glyphosate Degradation Performance and Indigenous Microbial Community Structure. — PubMed (NCBI E-utilities), 2022The mixed culture composed of Stenotrophomonas acidaminiphila and Ochrobactrum at a 2:1 ratio was able to achieve 79.41% degradation of the total petroleum hydrocarbons after 5 days.
Two types of microorganisms isolated from petroleum hydrocarbon pollutants: Degradation characteristics and metabolic pathways analysis of petroleum hydrocarbons. — PubMed (NCBI E-utilities), 2024After 5 days, the strain W02 exhibited the best degradation efficiency of C17, reaching 81.93%, whereas the lowest degradation efficiency for C21 (29.97%).
Two types of microorganisms isolated from petroleum hydrocarbon pollutants: Degradation characteristics and metabolic pathways analysis of petroleum hydrocarbons — PubMed Central (open access), 2024Notably, alginate bead-encapsulated UB08 achieved 100% Pb removal within 2 days, significantly better than immobilization on water hyacinth (100% at 5 days) and ceramic rings (71.51% at 7 days).
Scaling EPS yield and bio-carrier application of <i>Bacillus paralicheniformis</i> UB08 for rapid lead remediation. — Europe PMC, 2026The mixed culture composed of Stenotrophomonas acidaminiphila and Ochrobactrum at a 2:1 ratio was able to achieve 79.41% degradation of the total petroleum hydrocarbons after 5 days.
Two types of microorganisms isolated from petroleum hydrocarbon pollutants: Degradation characteristics and metabolic pathways analysis of petroleum hydrocarbons. — Europe PMC, 2024The mixed culture composed of Stenotrophomonas acidaminiphila and Ochrobactrum at a 2:1 ratio was able to achieve 79.41% degradation of the total petroleum hydrocarbons after 5 days.
Two types of microorganisms isolated from petroleum hydrocarbon pollutants: Degradation characteristics and metabolic pathways analysis of petroleum hydrocarbons. — DOAJ, 2024
Other values reported (4)
-
48 h
Evidence (6)
Under the optimized conditions, approximately 88% of DBP was degraded within 48 h and five metabolites were detected.
Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with Citrobacter freundii CD-9 to Degrade Fenvalerate. — PubMed (NCBI E-utilities), 2022A lag phase in decolorization of up to 48 h of incubation was observed, which may be attributed to the delay in growth of a member of the consortium responsible for causing reduction of the chromophore group of the dye and thus the decolorization of the medium.
Genome analysis to decipher syntrophy in the bacterial consortium ‘SCP’ for azo dye degradation — PubMed Central (open access), 2021In both cases, irradiated cells were serially diluted (10-fold) in normal saline (0.90% NaCl) and plated on trypticase soy agar (TSA) by the spread plate technique and incubated at 30 °C for 48 h in the dark to obtain viable cells.
Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila — PubMed Central (open access), 2022The plates were incubated at 30 °C for 48 h.
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — PubMed Central (open access), 2019Under the optimized conditions, approximately 88% of DBP was degraded within 48 h and five metabolites were detected.
Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with <i>Citrobacter freundii</i> CD-9 to Degrade Fenvalerate. — Europe PMC, 2022Notably, alginate bead-encapsulated UB08 achieved 100% Pb removal within 2 days, significantly better than immobilization on water hyacinth (100% at 5 days) and ceramic rings (71.51% at 7 days).
Scaling EPS yield and bio-carrier application of <i>Bacillus paralicheniformis</i> UB08 for rapid lead remediation. — Europe PMC, 2026
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36 h
Evidence (4)
The preparation was incubated at room temperature (28 ± 2 °C) on shaker incubator (Gallenkamp, UK) agitating at 150 rpm for 36 h.
Overproduction of a thermo-stable halo-alkaline protease on agro-waste-based optimized medium through alternate combinatorial random mutagenesis of Stenotrophomonas acidaminiphila — PubMed Central (open access), 2022S. maltophilia H3-1 biodegraded 93.3% of α-CBT-diol (300 mg/L) within 36 h when grown under optimized culture conditions, including a temperature of 40 °C, pH of 8, 2 g/L maltose, and 2 g/L ammonium sulfate.
Bioconversion of Alpha-Cembratriene-4,6-diol into High-Value Compound Farnesal Through Employment of a Novel <i>Stenotrophomonas maltophilia</i> H3-1 Strain. — Europe PMC, 2025The study also selected K2HPO4/KH2PO4 (2:1) and 108 cfu/mL as most appropriate phosphate combination and inoculum size respectively for maximum release of protease within the fermentation time of 36 h.
Evaluation of the Effects of Nutritional and Environmental Parameters on Extracellular Protease Production by Stenotrophomonas acidaminiphila Strain BPE4 — OpenAlex, 2018The study also selected K2HPO4/KH2PO4 (2:1) and 108 cfu/mL as most appropriate phosphate combination and inoculum size respectively for maximum release of protease within the fermentation time of 36 h.
Evaluation of the Effects of Nutritional and Environmental Parameters on Extracellular Protease Production by Stenotrophomonas acidaminiphila Strain BPE4 — Zenodo, 2018
-
7 d
Evidence (3)
In 7 days, Sten. acidaminiphila biofilm culture efficiently degraded 71·1 ± 3·1% and 40·2 ± 2·4% of phenanthrene and pyrene, respectively, whereas 38·7 ± 2·5% of phenanthrene and 29·7 ± 1% of pyrene degradation was observed in planktonic culture.
Characterization of Stenotrophomonas acidaminiphila NCW-702 biofilm for implication in the degradation of polycyclic aromatic hydrocarbons. — PubMed (NCBI E-utilities), 2014Then, the mixture-containing Erlenmeyer flask was incubated in a rotary shaker at 160 r.p.m. and 30 °C for 7 days.
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — PubMed Central (open access), 2019Notably, alginate bead-encapsulated UB08 achieved 100% Pb removal within 2 days, significantly better than immobilization on water hyacinth (100% at 5 days) and ceramic rings (71.51% at 7 days).
Scaling EPS yield and bio-carrier application of <i>Bacillus paralicheniformis</i> UB08 for rapid lead remediation. — Europe PMC, 2026
-
96 h
Evidence (4)
At initial concentrations of 50, 100, 200 and 300 mg l -1 , it degraded 91.5%, 89.4%, 86.5% and 83.5% of chlorothalonil after 96 h of inoculation under optimum conditions (30 °C and pH 7.0).
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil. — PubMed (NCBI E-utilities), 2019At initial concentrations of 50, 100, 200 and 300 mg l -1 , it degraded 91.5%, 89.4%, 86.5% and 83.5% of chlorothalonil after 96 h of inoculation under optimum conditions (30 °C and pH 7.0).
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — Crossref, 2019At initial concentrations of 50, 100, 200 and 300 mg l-1, it degraded 91.5%, 89.4%, 86.5% and 83.5% of chlorothalonil after 96 h of inoculation under optimum conditions (30 °C and pH 7.0).
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — DOAJ, 2019At initial concentrations of 50, 100, 200, 300 mg l-1, it degraded 91.5%, 89.4%, 86.5 and 83.5% of chlorothalonil after 96 h of inoculation under optimum conditions (30 °C and pH 7.0), respectively.
Data from: Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — Zenodo, 2019
Sources disagree: "5 d" (score 8.18) versus "48 h" (score 6.34). Both are shown; a reviewer should decide.
4 Colony morphology
lowThe colonies of strain BJ1 on LB plate were round with edge neat, uplifted surface, opaque and yellow in colour.
Evidence (1)
The colonies of strain BJ1 on LB plate were round with edge neat, uplifted surface, opaque and yellow in colour.
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — PubMed Central (open access), 2019
Other values reported (1)
-
For example, in the dilution of 10 -6 , 65 to 57 colony forming units (CFUs) were observed in the entire sample, equivalent to 1.2 × 10 8 bacteria per gram of the soil.
Evidence (1)
For example, in the dilution of 10 -6 , 65 to 57 colony forming units (CFUs) were observed in the entire sample, equivalent to 1.2 × 10 8 bacteria per gram of the soil.
Diuron degradation by bacteria from soil of sugarcane crops — PubMed Central (open access), 2017
5 Gram character
highGram-negative
Evidence (7)
Stenotrophomonas acidaminiphila , the most recent reported species in genus Stenotrophomonas , is a relatively rare bacteria and is an aerobic, glucose non-fermentative, Gram-negative bacterium.
Genome-wide analysis reveals the emergence of multidrug resistant Stenotrophomonas acidaminiphila strain SINDOREI isolated from a patient with sepsis. — PubMed (NCBI E-utilities), 2022Stenotrophomonas acidaminiphila is an aerobic, glucose non-fermentative, Gram-negative bacterium that been isolated from various environmental sources, particularly aquatic ecosystems.
Genome Sequencing and Comparative Analysis of Stenotrophomonas acidaminiphila Reveal Evolutionary Insights Into Sulfamethoxazole Resistance. — PubMed (NCBI E-utilities), 2018Cells were gram-negative, motile, non-sporulating, straight to curved rods with a polar flagellum.
Stenotrophomonas acidaminiphila sp. nov., a strictly aerobic bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor. — PubMed (NCBI E-utilities), 2002A total of 400 bacteria were isolated, with 68% being Gram-positive and 32% Gram-negative.
Diuron degradation by bacteria from soil of sugarcane crops. — PubMed (NCBI E-utilities), 2017A Gram-stain-negative, motile, aerobic bacterial strain, designated MJ03(T), was isolated from sewage and was characterized taxonomically by using a polyphasic approach.
Stenotrophomonas daejeonensis sp. nov., isolated from sewage. — PubMed (NCBI E-utilities), 2011A Gram-stain-negative, motile, aerobic bacterial strain, designated MJ03(T), was isolated from sewage and was characterized taxonomically by using a polyphasic approach.
Stenotrophomonas daejeonensis sp. nov., isolated from sewage. — PubMed (NCBI E-utilities), 2011Inferred from NCBI Taxonomy lineage: cellular organisms; Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Lysobacterales; Lysobacteraceae; Stenotrophomonas
NCBI Taxonomy lineage — NCBI Taxonomy, 2026
Other values reported (1)
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Gram-positive
Evidence (4)
A total of 400 bacteria were isolated, with 68% being Gram-positive and 32% Gram-negative.
Diuron degradation by bacteria from soil of sugarcane crops. — PubMed (NCBI E-utilities), 2017Of the 400 isolated strains, 68% were Gram-positive bacteria (GP) and 32% Gram-negative (GN) ( Table 1 ).
Diuron degradation by bacteria from soil of sugarcane crops — PubMed Central (open access), 2017Cultures that developed after inoculating vegetative cells were characterized by: (1) resistance to physical factors and sterilization procedures; (2) high antimicrobial activity with respect to some gram-positive and gram-negative bacteria; (3) resistance to polypeptide antibiotics; (4) the presence of an easily detaching S-layer on the cell surface; (5) the ability to secret outer membrane vesicles into the intercellular space; and (6) formation of S-layerderived tubular st
Cytophysiological Characteristics of the Vegetative and Dormant Cells of Stenotrophomonas sp. Strain FM3, a Bacterium Isolated from the Skin of a Xenopus laevis Frog — OpenAlex, 2018A total of 400 bacteria were isolated, with 68% being Gram-positive and 32% Gram-negative.
Diuron degradation by bacteria from soil of sugarcane crops — DOAJ, 2017
6 Biochemical tests for identification
high-
16S rRNA gene sequencing
Evidence (6)
The morphological, biochemical and genetic characteristics based on 16S rDNA sequence homology and phylogenetic analysis confirmed the isolate as Stenotrophomonas acidaminiphila strain JS-1.
Isolation and characterization of butachlor-catabolizing bacterial strain Stenotrophomonas acidaminiphila JS-1 from soil and assessment of its biodegradation potential. — PubMed (NCBI E-utilities), 2010Using 16S rDNA gene sequencing, morphological and biological characteristics, we identified the strain BJ1 as Stenotrophomonas acidaminiphila .
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil. — PubMed (NCBI E-utilities), 2019Based on their morphological, physiological and biochemical characteristics, together with 16S rDNA gene sequence analyses, bacteria were identified as Stenotrophomonas acidaminiphila (2#), Pseudomonas aeruginosa (9#) and Delftia tsuruhatensis (12#).
Characterization of cadmium-resistant bacteria and their potential for reducing accumulation of cadmium in rice grains. — PubMed (NCBI E-utilities), 2016The morphological, biochemical and genetic characteristics based on 16S rDNA sequence homology and phylogenetic analysis confirmed the isolate as Stenotrophomonas acidaminiphila strain JS-1.
Isolation and characterization of butachlor-catabolizing bacterial strain Stenotrophomonas acidaminiphila JS-1 from soil and assessment of its biodegradation potential. — PubMed (NCBI E-utilities), 2010The isolates were identified by 16S rRNA gene sequencing and phylogenetic analysis.
Scaling EPS yield and bio-carrier application of <i>Bacillus paralicheniformis</i> UB08 for rapid lead remediation. — Europe PMC, 2026Based on 16S rRNA gene sequencing, all three isolated strains were identified as belonging to the Klebsiella species.
Biodegradation of Microcystins by Aquatic Bacteria <i>Klebsiella</i> spp. Isolated from Lake Kasumigaura. — Europe PMC, 2025
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Whole-genome sequencing / ANI — reported positive
Evidence (4)
Whole genome sequencing (WGS) was used to identify all AR genes present in isolates.
Detection of Carbapenem-Resistant Bacteria in Skilled Nursing Facility Wastewater. — Europe PMC, 2026Whole-genome sequencing-based resistome characterization and phenotypic assessment of antibiotic resistance in Stenotrophomonas acidaminiphila K2-T isolated through polyethylene enrichment.
Whole-genome sequencing-based resistome characterization and phenotypic assessment of antibiotic resistance in Stenotrophomonas acidaminiphila K2-T isolated through polyethylene enrichment — Crossref, 2026Values for genomic average nucleotide identity (ANI; <95%) and digital DNA-DNA hybridization (dDDH; < 70%) indicated that strains CPCC 101365T and CPCC 101269T were well-differentiated from validly described Stenotrophomonas species, while strain CPCC 101426 shared high ANI (97.7%) and dDDH (78.3%) identity with its closest phylogenetic neighbor, Stenotrophomonas koreensis JCM 13256T.
Characterization of three Stenotrophomonas strains isolated from different ecosystems and proposal of Stenotrophomonas mori sp. nov. and Stenotrophomonas lacuserhaii sp. nov. — OpenAlex, 2022Based on phenotypic differences, chemotaxonomic properties, ANI and dDDH identity values, and phylogenetic analyses, two novel Stenotrophomonas species are proposed for the strains identified here.
Characterization of three Stenotrophomonas strains isolated from different ecosystems and proposal of Stenotrophomonas mori sp. nov. and Stenotrophomonas lacuserhaii sp. nov. — OpenAlex, 2022
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MALDI-TOF MS
Evidence (4)
A hybrid process for 2,4-dichlorophenoxy acetic acid herbicidal treatment and its microbial identification by MALDI-TOF mass spectrometry..
A hybrid process for 2,4-dichlorophenoxy acetic acid herbicidal treatment and its microbial identification by MALDI-TOF mass spectrometry. — PubMed (NCBI E-utilities), 2020Pre-treatment led to a quick decrease in concentration of 2,4-D and COD during the biological process: a 78.79 ± 0.30% COD removal and 38.23 ± 3.12% 2,4-D elimination was measured after 5760 min in A-BR, and 80.89 ± 0.81% COD and 81.36 ± 1.37% 2,4-D removal was achieved after 2880 min in P-B. For species identification using matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF)-TOF/MS equipment, Aeromonas eucrenophila , Stenotrophomonas acidaminiphila , Ral
A hybrid process for 2,4-dichlorophenoxy acetic acid herbicidal treatment and its microbial identification by MALDI-TOF mass spectrometry. — PubMed (NCBI E-utilities), 2020Results Bacteria (n = 209 isolates) were identified using MALDI-TOF and included >20 different genera.
Detection of Carbapenem-Resistant Bacteria in Skilled Nursing Facility Wastewater. — Europe PMC, 2026Showing highly similar with S. maltophilia, 704A1T also displayed distinct characteristic peaks in fatty acid profiling and MALDI-TOF analysis.
Stenotrophomonas tuberculopleuritidis sp. nov., a novel pathogenic Stenotrophomonas species isolated from tuberculous pleurisy patient — OpenAlex, 2025
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Indole
Evidence (6)
The strain JS-1 in stationary phase of culture also produced 21.0 microg ml(-1) of growth hormone indole acetic acid (IAA) in the presence of 500 microg ml(-1) of tryptophan.
Isolation and characterization of butachlor-catabolizing bacterial strain Stenotrophomonas acidaminiphila JS-1 from soil and assessment of its biodegradation potential. — PubMed (NCBI E-utilities), 2010All the rhizobacteria showed plant growth promoting traits such as production of siderophores, indole acetic acid (IAA), phosphate solubilization and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity.
Copper-tolerant rhizosphere bacteria-characterization and assessment of plant growth promoting factors. — PubMed (NCBI E-utilities), 2017The strain JS-1 in stationary phase of culture also produced 21.0 microg ml(-1) of growth hormone indole acetic acid (IAA) in the presence of 500 microg ml(-1) of tryptophan.
Isolation and characterization of butachlor-catabolizing bacterial strain Stenotrophomonas acidaminiphila JS-1 from soil and assessment of its biodegradation potential. — PubMed (NCBI E-utilities), 2010It did not produce hydrogen sulfide and indole while it was able to use glucose, propionate, malic acid, pyruvic acid and acidic amino acid as sole carbon sources.
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — PubMed Central (open access), 2019The strain JS-1 in stationary phase of culture also produced 21.0 microg ml(-1) of growth hormone indole acetic acid (IAA) in the presence of 500 microg ml(-1) of tryptophan.
Isolation and characterization of butachlor-catabolizing bacterial strain Stenotrophomonas acidaminiphila JS-1 from soil and assessment of its biodegradation potential — OpenAlex, 2010Genes encoding enzymes involved in the biosynthesis of indole-3-acetic acid (IAA) and siderophores were identified in the genomes of the three isolates, suggesting that these strains might serve roles as plant-growth promoting microorganisms.
Characterization of three Stenotrophomonas strains isolated from different ecosystems and proposal of Stenotrophomonas mori sp. nov. and Stenotrophomonas lacuserhaii sp. nov. — OpenAlex, 2022
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Motility — reported negative
Evidence (5)
Cells were gram-negative, motile, non-sporulating, straight to curved rods with a polar flagellum.
Stenotrophomonas acidaminiphila sp. nov., a strictly aerobic bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor. — PubMed (NCBI E-utilities), 2002A Gram-stain-negative, motile, aerobic bacterial strain, designated MJ03(T), was isolated from sewage and was characterized taxonomically by using a polyphasic approach.
Stenotrophomonas daejeonensis sp. nov., isolated from sewage. — PubMed (NCBI E-utilities), 2011A Gram-stain-negative, motile, aerobic bacterial strain, designated MJ03(T), was isolated from sewage and was characterized taxonomically by using a polyphasic approach.
Stenotrophomonas daejeonensis sp. nov., isolated from sewage. — PubMed (NCBI E-utilities), 2011Strain ASS1, a motile Gram-negative rod, was isolated from crude oil-contaminated soil in Tabasco, Mexico, and initial 16S rRNA gene sequencing indicated affiliation with Stenotrophomonas , warranting full polyphasic characterization.
<i>Stenotrophomonas oleivorans</i> sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from crude oil-contaminated soil in Tabasco, Mexico. — Europe PMC, 2026Stenotrophomonas acidaminiphila is a strictly aerobic, gram-negative, mesophilic, non-spore-forming and motile bacterium from the genus Stenotrophomonas which has been isolated from industrial waste water in Mexico.
Stenotrophomonas acidaminiphila — Wikipedia, 2026
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Short-chain fatty acid profile (GLC)
Evidence (3)
Metabolites formed as a result of fipronil degradation were characterized with gas liquid chromatograph.
Degradation of fipronil by Stenotrophomonas acidaminiphila isolated from rhizospheric soil of Zea mays. — PubMed (NCBI E-utilities), 2016Metabolites formed as a result of fipronil degradation were characterized with gas liquid chromatograph.
Degradation of fipronil by Stenotrophomonas acidaminiphila isolated from rhizospheric soil of Zea mays. — PubMed (NCBI E-utilities), 2016Metabolites formed as a result of fipronil degradation were characterized with gas liquid chromatograph.
Degradation of fipronil by Stenotrophomonas acidaminiphila isolated from rhizospheric soil of Zea mays — OpenAlex, 2016
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Cellulose (CMC) hydrolysis
Evidence (2)
This study investigates the isolation and characterization of novel lignocellulolytic bacterial strains capable of efficiently degrading paddy straw, thereby contributing to sustainable carbon management.
Bio decomposition of paddy straw waste using laccase producing novel soil bacterial isolates: An eco-friendly and sustainable approach. — Europe PMC, 2026Enzyme kinetic studies indicated optimal laccase activity at temperatures of 45-55 °C and pH levels of 4-7, with isolates TR32, SH2A, and AK17 producing laccase activities exceeding 90 U/L. Molecular characterization via 16 S rRNA sequencing identified these isolates as belonging to genera such as Pseudomonas, Enterobacter, Acinetobacter, and Klebsiella, known for their lignocellulolytic enzyme production.
Bio decomposition of paddy straw waste using laccase producing novel soil bacterial isolates: An eco-friendly and sustainable approach. — Europe PMC, 2026
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Antibiotic susceptibility profile
Evidence (2)
Antibiotic susceptibility and physiological characteristics were determined.
Stenotrophomonas acidaminiphila sp. nov., a strictly aerobic bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor. — PubMed (NCBI E-utilities), 2002Methods Systematical evaluations including phenotypic and biochemical characteristics, antibiotic susceptibility, genomic sequencing were conducted.
Stenotrophomonas tuberculopleuritidis sp. nov., a novel pathogenic Stenotrophomonas species isolated from tuberculous pleurisy patient — OpenAlex, 2025
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Spore formation / stain — reported negative
Evidence (2)
Here, three Gram-stain negative, rod-shaped, non-spore-forming bacteria, designated as strains CPCC 101365T, CPCC 101269T, and CPCC 101426 were isolated from surface-sterilized medicinal plant roots of a mulberry plant in Chuxiong of the Yunnan Province, freshwater from Erhai Lake in the Yunnan Province, and sandy soils in the Badain Jaran desert in Inner Mongolia Autonomous Region, China, respectively.
Characterization of three Stenotrophomonas strains isolated from different ecosystems and proposal of Stenotrophomonas mori sp. nov. and Stenotrophomonas lacuserhaii sp. nov. — OpenAlex, 2022Stenotrophomonas acidaminiphila is a strictly aerobic, gram-negative, mesophilic, non-spore-forming and motile bacterium from the genus Stenotrophomonas which has been isolated from industrial waste water in Mexico.
Stenotrophomonas acidaminiphila — Wikipedia, 2026
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Catalase — reported positive
Evidence (1)
Biochemically, the strain was negative for oxidase, urease, nitrate reduction, hydrolysis starch, citrate utilization test and was positive for catalase and gelatin hydrolysis.
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — PubMed Central (open access), 2019
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Citrate utilisation — reported positive
Evidence (1)
Biochemically, the strain was negative for oxidase, urease, nitrate reduction, hydrolysis starch, citrate utilization test and was positive for catalase and gelatin hydrolysis.
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — PubMed Central (open access), 2019
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Urease — reported negative
Evidence (1)
Biochemically, the strain was negative for oxidase, urease, nitrate reduction, hydrolysis starch, citrate utilization test and was positive for catalase and gelatin hydrolysis.
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — PubMed Central (open access), 2019
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Nitrate reduction — reported negative
Evidence (1)
Biochemically, the strain was negative for oxidase, urease, nitrate reduction, hydrolysis starch, citrate utilization test and was positive for catalase and gelatin hydrolysis.
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — PubMed Central (open access), 2019
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Gelatin hydrolysis — reported positive
Evidence (1)
Biochemically, the strain was negative for oxidase, urease, nitrate reduction, hydrolysis starch, citrate utilization test and was positive for catalase and gelatin hydrolysis.
Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil — PubMed Central (open access), 2019
7 References used
59 sourcesCatalogues to check by hand
These have no open interface, or their terms forbid automated querying. The app prepares the query; you open it.
Scan log — what each database returned
| Database | Status | Records | Evidence | Time | Note |
|---|---|---|---|---|---|
| NCBI Taxonomy — lineage and accepted name | empty | 0 | 0 | 1357 ms | TaxID 128780 (species). |
| LPSN — nomenclatural status | skipped | 0 | 0 | 0 ms | Skipped — LPSN credentials not configured (free registration). |
| StrainInfo — strain cross-references | empty | 0 | 0 | 1323 ms | No response (endpoint may require a different path). |
| GBIF — name resolution | empty | 0 | 0 | 643 ms | Matched as Stenotrophomonas acidaminiphila A (ACCEPTED). |
| Wikidata — identifier bridge | empty | 0 | 0 | 1217 ms | Item Q10374156 matched. |
| DSMZ MediaDive — curated growth media | empty | 0 | 0 | 800 ms | No media entries matched this name in MediaDive. |
| BacDive — curated strain phenotypes | skipped | 0 | 0 | 0 ms | Skipped — BacDive credentials not configured. This is the single highest-value source; registration is free. |
| PubMed — culture and cultivation query | hit | 25 | 35 | 2517 ms | 25 abstracts retrieved (culture query). |
| PubMed — morphology and identification query | hit | 6 | 14 | 2479 ms | 6 abstracts retrieved (pheno query). |
| PubMed Central — open-access Methods sections | hit | 5 | 38 | 8812 ms | 5 open-access full texts mined. |
| Europe PMC — abstract search | hit | 25 | 38 | 1249 ms | 25 records from Europe PMC. |
| Europe PMC — full-text mining | empty | 0 | 0 | 3000 ms | 0 full texts mined for Methods detail. |
| Crossref — DOI metadata | hit | 25 | 4 | 943 ms | 25 DOI records (abstracts only where deposited). |
| OpenAlex — open scholarly graph | hit | 25 | 28 | 2643 ms | 25 works (inverted abstracts reconstructed locally). |
| Semantic Scholar — abstracts | empty | 0 | 0 | 1147 ms | Rate-limited or unavailable (keyless access is throttled). |
| DOAJ — open-access journals | hit | 17 | 12 | 567 ms | 17 open-access articles. |
| OpenAIRE — repository publications | empty | 0 | 0 | 14009 ms | 0 repository publications. |
| SciELO — regional journals | empty | 0 | 0 | 573 ms | No parseable SciELO response (their JSON output is unstable; a deep link is still provided). |
| NCBI Bookshelf — reference texts | empty | 0 | 0 | 645 ms | No reference-text chapters matched. |
| CORE — aggregated repositories | skipped | 0 | 0 | 0 ms | Skipped — no API key configured. |
| Springer Nature — publisher metadata | skipped | 0 | 0 | 0 ms | Skipped — no API key configured. |
| ScienceDirect — publisher metadata | skipped | 0 | 0 | 0 ms | Skipped — no API key configured. |
| bioRxiv / medRxiv — preprints | hit | 2 | 2 | 902 ms | 2 preprints (not peer reviewed — weighted down). |
| Zenodo — datasets and protocols | hit | 2 | 7 | 920 ms | 2 deposits (datasets, theses, protocols). |
| Wikipedia — orientation only | hit | 1 | 3 | 848 ms | Article retrieved (lowest weight). |
| ATCC — deep link | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |
| NCTC / UKHSA — deep link | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |
| Google Scholar — deep link (not crawled) | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |
| protocols.io — deep link | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |
| ResearchGate — deep link | empty | 0 | 0 | 0 ms | Deep link prepared — open manually. |